GLYCOPROTEINS CONTAINING PHOSPHOGLUCOSE IN NEURAL RETINA

神经视网膜中含有磷酸葡萄糖的糖蛋白

• 批准号: 3263303
• 负责人: Richard Banfield Marchase
• 金额: $ 12.32万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-04-01 至 1990-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3263303
• 关键词: affinity chromatography; antibody specificity; autoradiography; binding proteins; cell adhesion; cell cell interaction; chick embryo; electron microscopy; enzyme linked immunosorbent assay; enzyme mechanism; gel electrophoresis; glucose; glycoproteins; immunofluorescence technique; immunoprecipitation; laboratory rabbit; laboratory rat; mannose; membrane proteins; monoclonal antibody; neuronal transport; oligosaccharides; phosphodiesterases; protein biosynthesis; protein sequence; radiotracer; retina; retinal ganglion; synapses; visual pathways

Glycoprotein-associated oligosaccharides from embryonic chick neural retina which contain glucose in phosphodiester-linkage to mannose (ManPGlc) have recently been detected by our laboratory, as has an enzyme responsible for the synthesis of the phosphodiester and a group of peptides that specifically recognize them. This proposal seeks to investigate at a molecular level the changes these proteins undergo during development of the retina and to assess the roles these proteins may play in the physiologic functions of the visual system. Particular attention will be paid to the possibility that this unique oligosaccharide functions as a trafficking signal to segregate these glycoproteins for axoplasmic transport, and that once on the cell surface these glycoproteins function in interneuronal recognition. The specific aims of this proposal are: I. To pursue further studies of the glycoprotein acceptors in retina recognized in the phosphoglucosyl transferase reaction. Studies will include determinations of the specific activities of the phosphoglucosyltransferase and identification by autoradiographic analyses of gels of its acceptors in chick retina, tectum, and other parts of the CNS. II. To continue our characterization of ManPGlc binding proteins in embryonic chick neural retina. The proposed studies include a determination of the amino acid sequences of these peptides, and of their relationships to one another and to the previously described cell-surface protein ligatin. III. To determine the importance of ManPGlc-containing glycoproteins to cell-to-cell adhesion and, ultimately, to interneuronal synaptic specificity. We propose to pursue studies of intercellular recognition by monitoring the effects of exogenously adding these proteins themselves, their oligosaccharide analogs, or antibodies directed against them, to in vitro assays for intercellular adhesion. IV. To examine the possibility that oligosaccharides containing ManPGlc may be an important feature in the selection of certain glycoproteins for axoplasmic transport in retinal ganglion cells. Experiments in neural retina will determine if the ManPGlc binding proteins and/or glycoproteins carrying phosphodiester-linked glucose are axonally transported and, if so, to determine the importance of this oligosaccharide to this process.

来自胚胎鸡神经视网膜的糖蛋白相关寡糖 含有与甘露糖磷酸二酯键连接的葡萄糖 (ManPGlc) 最近我们的实验室检测到，有一种酶负责 磷酸二酯和一组肽的合成 特别认识他们。 该提案旨在调查 分子水平上这些蛋白质在发育过程中经历的变化 视网膜并评估这些蛋白质在视网膜中可能发挥的作用 视觉系统的生理功能。 将特别注意 归功于这种独特的寡糖作为 运输信号以分离这些糖蛋白用于轴浆 运输，一旦到达细胞表面，这些糖蛋白就会发挥作用 在神经元间识别中。 本提案的具体目的是： 一、进一步研究 视网膜中的糖蛋白受体在磷酸葡萄糖基中被识别 转移酶反应。 研究将包括具体的确定 磷酸葡萄糖基转移酶的活性及其鉴定 对其受体在小鸡视网膜、顶盖中的凝胶进行放射自显影分析， 和中枢神经系统的其他部分。 二.继续我们的表征 胚胎鸡神经视网膜中的 ManPGlc 结合蛋白。 拟议的 研究包括确定这些物质的氨基酸序列 肽，以及它们之间的关系以及它们与先前的肽的关系 描述了细胞表面蛋白连接素。 三．确定重要性 含有 ManPGlc 的糖蛋白对细胞间粘附的影响， 最终，神经元间突触特异性。 我们建议追求 通过监测细胞间识别的影响进行研究 外源添加这些蛋白质本身及其寡糖 类似物或针对它们的抗体，用于体外测定 细胞间粘附。 四．为了检验以下可能性 含有 ManPGlc 的寡糖可能是 选择某些糖蛋白进行视网膜轴浆运输 神经节细胞。 神经视网膜实验将确定 ManPGlc 是否 携带磷酸二酯连接的结合蛋白和/或糖蛋白 葡萄糖是轴突运输的，如果是这样，确定的重要性 这种低聚糖参与到这个过程中。