OPTICAL ROTATORY POWER OF BIOPOLYMERS

生物聚合物的旋光能力

• 批准号: 3268178
• 负责人: JEN T YANG
• 金额: $ 15.53万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-05-01 至 1989-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3268178
• 关键词: acetylcholinesterase; bacteriorhodopsins; bungarotoxins; chemical aggregate; circular dichroism; conformation; copper; detergents; hydropathy; ligands; liposomes; membrane lipids; membrane proteins; metalloproteins; nicotinic receptors; optical rotation; peptide structure; phospholipids; protein signal sequence; protein structure function; superoxide dismutase; synthetic peptide; thermodynamics

The long-range objective is to understand the structure-function relationship of a select few biopolymers by biophysical studies. The conformation of several polypeptides and proteins in solutions will be studied by circular dichroism together with other physicochemical methods. Our working hypothesis that the conformation of polypeptides in surfactant and lipid solutions is dictated by their primary structure will be further tested on amphipathic polypeptides (e.g. fragments of melittin and bacteriorhodopsin) and strongly hydrophobic polypeptides (e.g. signal peptides). Two new projects are initiated: one on two major proteins in cholinergic neurotransmission and the other on metal ion-cyclic peptide complexes. The conformation and enzymic activity of both the asymmetric (soluble) and globular (membrane-bound) forms of acetylcholinesterase and those of acetylcholine receptor and its subunits (Alpha2BetaGammaDelta) from Torpedo californica will be studied. The conformational changes, if any, of the receptor in the presence of agonists and antagonists will be determined. The conformation of reconstituted receptor and enzyme in lipid vesicles will be investigated by various physical methods. Second, a series of diketopiperazines such as c(His)2 and c(Cys-Met) and, later, cyclic hexapeptides will be synthesized. The coordination complexes of these compounds with metal ions such as Cu, Co, Ni, Mn and Fe mimick the active sites of several metalloproteins such as Cu/Zn superoxide dismutase, plastocyanin and azurin. The conformation and possible catalytic activity of these complexes will be related to and compared with those of the corresponding metalloproteins. The proteins and model compounds are chosen for their biological interest and health-related importance.

长期目标是理解结构功能 通过生物物理学研究选定的几种生物聚合物之间的关系。 这 溶液中几种多肽和蛋白质的构象将是 通过圆二色性和其他物理化学方法一起研究。 我们的工作假设是表面活性剂中多肽的构象 和脂质溶液由其一级结构决定将进一步 对两亲性多肽（例如蜂毒肽和 细菌视紫红质）和强疏水性多肽（例如信号 肽）。 启动了两个新项目：一个是关于两种主要蛋白质的项目 胆碱能神经传递和其他金属离子环肽 复合物。 两种不对称分子的构象和酶活性 （可溶性）和球状（膜结合）形式的乙酰胆碱酯酶和 乙酰胆碱受体及其亚基 (Alpha2BetaGammaDelta) 来自加州鱼雷的研究将被研究。 构象发生变化，如果 在存在激动剂和拮抗剂的情况下，任何受体都将是 决定。 脂质中重构受体和酶的构象 将通过各种物理方法研究囊泡。 第二，一个 系列二酮哌嗪，例如 c(His)2 和 c(Cys-Met)，以及后来的 将合成环状六肽。 的配位络合物 这些与金属离子（如 Cu、Co、Ni、Mn 和 Fe）的化合物模仿 几种金属蛋白的活性位点，例如铜/锌超氧化物歧化酶， 质体蓝蛋白和天青蛋白。 构象和可能的催化活性 这些复合体将与那些复合体相关并进行比较 相应的金属蛋白。 选择蛋白质和模型化合物 因其生物学意义和与健康相关的重要性。