New Photonic Materials from Genetically Engineered Bacteriorhodopsins

来自基因工程细菌视紫红质的新型光子材料

• 批准号: 9202209
• 负责人: Richard Needleman
• 金额: $ 55万
• 依托单位: Wayne State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-15 至 1996-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9202209&HistoricalAwards=false
• 关键词: New; Photonic; Materials; Genetically; Engineered

New Photonic Material from Genetically Engineered Bacteriorhodopsin. There exists a need for photonic materials that have superior photochromic, photovoltaic, or nonlinear optical (NLO) characteristics. This research will develop a new family of materials based on the protein bacteriorhodopsin (BR), a unique biological material which has promising photochromic, photooltaic, and nonlinear optical properties needed for device application (e.g. holographic recording films, associative holographic memories, spatial light modulators, etc.). To fully realize the potential of devices based on BR one must produce mutant BRs at a scale suitable for rapid analysis and fabrication and be able to screen these mutant Brs rapidly for important photochromic properties (second harmonic generation). Mutants construction from the gene coding for BR (bop) will use an expression system which has recently been developed. In this system mutant BR's are synthesized in their natural host and not in E. coli as is currently the custom. This method allows the rapid and facile production of large quantities of mutant proteins derived by site-directed mutagenesis. The photochromic properties, the photovoltaic properties, and the nonlinear optical properties of the BR variants will be assessed. This effort involves the close collaboration of investigators with expertise in three different areas: genetics (Richard Needleman). photochemistry (Janos Lanyi) and physics (George Rayfield). A strong industrial collaboration (Bend Research Inc, Bend Oregon) is also part of this program. %%% There exists a need for materials that have superior responses to light than man-made products currently in existence. This research will develop a new family of materials based on the protein bacteriorhodopsin (BR), a unique biological material which has promising light sensitive and photoelectric properties needed for device application (e.g. holographic recording films, associative holographic memories, spatial light modulators, etc.). To fully realize the potential of devices based on BR one must produce mutant Brs at a scale suitable for rapid analysis and fabrication to screen these mutant Brs for important photochromic properties. Mutants proteins will be obtained from the gene coding for BR inserted into a bacterial expression system involving the natural host; this method allows the rapid and facile production of large quantities of mutant proteins derived by site-directed mutagenesis. The photoelectronic properties of these light sensitive proteins will be assessed by investigators with expertise in three different areas: genetics, photochemistry, and physics. A strong industrial collaboration is also part of this program.

基于基因工程的新型光子材料 细菌视紫红质。 需要光子材料， 具有上级光致变色、光伏或非线性 光学（NLO）特性。 这项研究将开发一种新的 基于细菌视紫红质蛋白质的材料家族 (BR)一种独特的生物材料， 光致变色、光致变色和非线性光学性质 设备应用（例如全息记录）所需的 电影，关联全息记忆，空间光 调制器等）。充分发挥设备的潜力 基于BR，必须以适合于 快速分析和制造，并能够筛选这些突变体， BRS快速获得重要的光致变色性能（二次谐波 代）。从编码以下的基因构建突变体： BR（bop）将使用一种表达系统， 开发 在该系统中，突变体BR在它们的细胞中合成。 自然宿主，而非E.大肠杆菌是目前的习惯。 这种方法允许快速和容易地生产大量的 通过定点突变获得的突变蛋白的量 诱变 光致变色特性，光伏特性 性质，以及BR变体的非线性光学性质 将予以评估。 这项工作涉及以下方面的密切合作： 具有三个不同领域专长的研究人员：遗传学 （Richard Needleman）光化学（Janos Lanyi）和物理学 （乔治雷菲尔德）。 强有力的工业合作（弯曲 研究公司，弯曲俄勒冈州）也是这一计划的一部分。 %%% 存在对具有上级响应的材料的需要， 比现有的人造产品轻。 本研究 将在蛋白质的基础上开发一种新的材料家族， 细菌视紫红质（BR），一种独特的生物材料， 具有良好的光敏和光电性能 设备应用（例如全息记录）所需的 电影，关联全息记忆，空间光 调制器等）。充分发挥设备的潜力 基于BR，必须以适合于 快速分析和制造，以筛选这些突变的Brs， 重要的光致变色性能。突变蛋白将是 从插入细菌中的编码BR的基因获得 涉及天然宿主的表达系统;该方法允许 大量突变体快速而容易的生产 通过定点诱变得到的蛋白质。 光电子 这些光敏蛋白的性质将通过以下方法来评估： 研究人员在三个不同领域的专业知识：遗传学， 光化学和物理学。 强大的工业合作是 也是这个项目的一部分。