MASS SPECTROMETRIC SEQUENCING OF PROTEINS

蛋白质质谱测序

• 批准号: 3267884
• 负责人: KLAUS BIEMANN
• 金额: $ 20.34万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-01-01 至 1990-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3267884
• 关键词: DNA; Escherichia coli; aminoacid tRNA ligase; antineoplastics; calcium binding protein; chickens; collagen; gas chromatography; gas chromatography mass spectrometry; genetic translation; high performance liquid chromatography; mass spectrometry; molecular size; nucleic acid sequence

The development of mass spectrometric methods for the determination of the primary structure of proteins will be continued and applied to new proteins. The sensitivity of the reductive GCMS method for peptide sequencing will be improved by carrying out the derivatization in the gas phase. Fast-atom bombardment and tandem mass spectrometry will be used to determine peptide sequences. Laser induced fragmentation will be investigated for this purpose. These methodologies will be used to determine the structure of a series of thioredoxins, ubiquitous enzymes present in all organisms from bacteria to man; of a viral coat protein and other proteins. Specific enzymatic and chemical peptide bond cleavage methods will be explored for their specific utility in these studies.

质谱法的发展用于确定 蛋白质的主要结构将继续并应用于新的 蛋白质。 还原性GCMS方法对肽的灵敏度 通过在气体中进行衍生化来改善测序 阶段。 快速原子轰炸和串联质谱法将用于 确定肽序列。 激光诱导的碎片将是 为此目的进行了调查。 这些方法将用于确定一系列的结构 硫蛋白，无处不在的酶，从细菌到 男人;病毒涂料蛋白和其他蛋白质的。 特定的酶促和化学肽键裂解方法将是 在这些研究中探索了他们的特定效用。