ENDOTHELIAL-LYMPHOCYTE INTERACTIONS IN THE RETINA

参与葡萄膜道免疫的白细胞 炎症必须附着并穿过内皮细胞才能到达 血管周围组织，在那里它们会引起疾病。当地的因素 调节眼内淋巴细胞反应，包括淋巴细胞抑制剂 玻璃体和血管周围的神经胶质细胞均有增殖。 细胞。血管内皮细胞生长的抑制因子已在 玻璃体，这被认为是正常的部分原因 玻璃体无血管，其中之一似乎是转化生长因子β。工作量很大 提示内皮细胞可以刺激淋巴细胞增殖和 过程抗原。该项目旨在分析 血管内皮细胞、淋巴细胞功能和抑制物 与世隔绝。它包括a)淋巴细胞抑制物的纯化，以及 确定其是否与内皮抑制物相同，b)确定 在淋巴细胞进入组织的过程处于什么状态时， 抑制物起作用的淋巴细胞的分化和增殖，c) 确定它是直接作用于淋巴细胞还是通过中介作用 通过刺激淋巴细胞的细胞，如单核细胞或内皮细胞 细胞，d)确定该因子在体内和体内是否有效 组织培养，e)确定淋巴细胞的哪些亚群 影响因素，f)确定纯化的内皮细胞抑制物 细胞生长也有类似的影响。长期目标是使用这些 计划可以抑制局部免疫反应发生的治疗方法 免疫介导性眼病。

The white blood cells which are involved in uveal tract immune-mediated inflammation must adhere to and traverse endothelium to reach the perivascular tissues, where they cause disease. Local factors which modulate lymphocyte responses in eye, including inhibitors of lymphocyte proliferation have been demonstrated in vitreous and in perivascular glial cells. Inhibitors of endothelial cell growth have been demonstrated in vitreous, which are believed to be partly responsible for the normal avascularity of vitreous, one of these appears to be TGF beta. Much work suggests endothelial cells can stimulate lymphocyte proliferation and process antigen. This project aims at analyzing relationships between endothelial cells, lymphocyte function, and the inhibitors previously isolated. It includes a) purification of the lymphocyte inhibitors, and determining if it is the same as the endothelial inhibitor, b) determining at which state of the processes of lymphocyte entry into the tissue, differentiation, and proliferation of lymphocytes the inhibitors works, c) determining whether it is a direct effect on lymphocytes or is mediated through cells which stimulate lymphocytes such as monocytes or endothelial cells, d) determining whether the factor is effective in vivo as well as in tissue culture, e) determining which subpopulations of lymphocytes the factor affects, f) determining whether purified inhibitors of endothelial cell growth have similar effects. The long-term objective is to use these to plan treatments which can suppress the local immune response occurring in immune-mediated ocular disease.