METABOLISM OF OCULAR SURFACE EPITHELIUM

眼表上皮的代谢

• 批准号: 3262253
• 负责人: RICHARD A THOFT
• 金额: $ 23.81万
• 依托单位: EYE AND EAR INSTITUTE OF PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-06-01 至 1993-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3262253
• 关键词: autoradiography; biopsy; cellular pathology; collagen; conjunctiva; cornea disorder; corneal epithelium; cytotoxicity; disease /disorder model; electron microscopy; eye disorder; eye disorder diagnosis; histochemistry /cytochemistry; human tissue; hypersensitivity; immunochemistry; immunosuppression; inflammation; laboratory mouse; laboratory rabbit; membrane activity; mucopolysaccharides; organ culture; stress; vitamin A deficiency

Ocular surface diseases affecting the corneal and conjunctival epithelia and immediately subjacent tissues are among the most difficult external eye diseases to treat. The overall goals of this grant are improve our understanding of the normal and diseased conjunctiva with the eventual aim being to define and manipulate some of the factors responsible for ocular surface disease. Hyperproliferation of the conjunctival epithelium has been demonstrated in ocular surface diseases such as ocular cicatricial pemphigoid (OCP) and vitamin A deficiency. It is unknown whether this is a general response of the tissue to inflammation or specific to disease. Therefore, one of the aims of this application is to investigate the role of inflammation in differentiation of the ocular surface using both animal models and patients. Ocular surface diseases are frequently associated with conjunctival scarring and subepithelial fibrosis; however, little attention has been paid to the biology of the conjunctival subepithelial cells and matrix. In this study, we will study conjunctival fibrocytes in culture, concentrating both on their growth characteristics and on the production of collagen and glycosaminoglycans by these cells from normal and diseased eyes. Furthermore, we will assess the subepithelial matrix and cells immunohistochemically in tissue from normal and diseased eyes. Conjunctival biopsy and impression cytology are two methods of obtaining samples of conjunctiva for analysis. Impression cytology is less invasive than conjunctival biopsy, but may not provide as much information. By comparing these methods with respect to goblet cell frequency, mitotic rate, and squamous metaplasia, we propose to determine whether it is possible to use impression cytology rather than conjunctival biopsy to define types of ocular surface disease. Finally, we will measure the effect of systemic cytotoxic immunosuppression on several defined characteristics of OCP to determine the response to therapy.

影响角膜和结膜的眼表疾病 上皮细胞和直接皮下组织是最常见的 难治的外部眼病治疗。这一总体目标 赠款是提高我们的理解正常和疾病 最终目的是为了定义和操作 一些导致眼表疾病的因素。 结膜上皮过度增生已被发现 在眼表疾病如眼瘢痕性 类天疱疮（OCP）和维生素A缺乏症。 尚不清楚 这是否是组织对炎症的一般反应， 具体到疾病。 因此，本申请的目的之一是 目的是研究炎症在肿瘤细胞分化中的作用， 使用动物模型和患者的眼表面。 眼表疾病通常与 结膜瘢痕和上皮下纤维化;然而， 人们已经注意到结膜炎的生物学 上皮下细胞和基质。 在这项研究中，我们将研究 结膜纤维细胞培养，集中在他们的 生长特性和对胶原蛋白的生产， 这些细胞从正常和患病的眼睛。 此外，我们将评估上皮下基质和细胞 在正常和患病眼睛的组织中进行化学分析。 结膜活检和印模细胞学检查是诊断结膜炎的两种方法。 获得结膜样品用于分析。 印象 细胞学检查比结膜活检侵入性小，但可能 提供尽可能多的信息 通过比较这些方法， 杯状细胞频率、有丝分裂率和鳞状细胞 化生，我们建议确定是否有可能使用 印模细胞学检查而不是结膜活检来确定 眼表疾病的类型。 最后，我们将测量全身细胞毒性的影响， 免疫抑制对OCP几个确定特征的影响， 确定对治疗的反应。