STRUCTURE AND FUNCTION OF HEART MEMBRANE PROTEINS

本研究的总体目标是阐明这一途径。 电子转移与质子能量传递机制 线粒体细胞色素Bc1复合体中的易位 呼吸链。在即将到来的项目期内进行试验 将集中在识别质子转移反应和 参与这些反应的氧化还原蛋白。 实验的具体目的是定位泛醌和/或 泛喹酚结合部位，推测位于Bc1的铁-硫蛋白上 质子与电子同时释放的复合体 调职。将进行相关研究，以测试类似的 细胞色素b上的对苯二酚/对苯二酚结合部位 实验将测试细胞色素b是否是跨膜的， 氧化还原连接质子泵。 这些研究将在线粒体和两种 好氧细菌在回答问题方面提供了独特的优势 这些问题不是很容易通过实验来回答的 线粒体。所采用的方法包括膜提纯 呼吸链复合体进入磷脂小泡，与光亲和 贴标签。

The overall objective of this research is to elucidate the pathway of electron transfer and the mechanism of energy transducing proton translocation in the cytochrome bc1 complex of the mitochondrial respiratory chain. During the forth-coming project period experimentation will be focused on identifying the proton translocation reactions and the redox proteins involved in those reactions. The specific aims of the experimentation are to locate a ubiquinone and/or ubiquinol binding site, presumably on the iron-sulfur protein of the bc1 complex, from which protons are released coincident with electron transfer. Related studies will be conducted to test for a similar quinone/quinol binding site on cytochrome b. A second aim of the experimentation will be to test whether cytochrome b is a transmembranous, redox linked proton pump. These studies will be carried out with mitochondria and with two species of aerobic bacteria which offer unique advantages to answering aspects of these questions which are not as readily answered by experimentation with mitochondria. The methods to be used include purification of membranous respiratory chain complexes into phospholipid vesicles, and photoaffinity labeling.