Mechanism of Respiration and Energy Transduction

呼吸和能量转换机制

• 批准号: 7152913
• 负责人: BERNARD L TRUMPOWER
• 金额: $ 57.4万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-06-01 至 2008-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7152913
• 关键词: Address; Affect; Amino Acids; Bacteria; Binding; Biochemical; Cell Respiration; Cell membrane; Complex; Condition; Cytochrome bc1 Complex; Cytochrome c Group; Cytochromes; Electron Transport; Enzymes; Eukaryotic Cell; Heme; Hydroquinones; Inner mitochondrial membrane; Iron; Ligand Binding; Ligands; Link; Localized; Measures; Membrane; Methods; Mitochondria; Molecular Genetics; Multienzyme Complexes; Mutation; Nitrogen Fixation; Organism; Oxidation-Reduction; Oxygen; Pathway interactions; Protons; Rate; Reaction; Research; Research Personnel; Research Project Grants; Resistance; Respiration; Saccharomyces cerevisiae; Signal Transduction; Site; Site-Directed Mutagenesis; Sulfur; Testing; Ubiquinone; Water; Yeasts; antimycin; base; cytochrome c; denitrification; enzyme mechanism; monomer; mutant; oxidation; programs; reaction rate; semiquinone; ubiquinol; ubisemiquinone

The cytochrome bc1 complex is an energy-transducing enzyme that participates in cell respiration in oxygen utilizing eukaryotic cells and is located in the inner mitochondrial membrane. A similar bc1 complex is located in the plasma membrane of many bacteria, where it takes part in respiration, denitrification, nitrogen fixation, and cyclic photosynthetic electron transfer, depending on the species. In all of these organisms the bc1 complex oxidizes a membrane-localized quinol and reduces a water-soluble, c-type cytochrome and links this electron transfer reaction to translocation of protons across the membrane in which the bc1 complex resides. The bc1 complexes from mitochondria of several species have been crystallized and the mechanism of the enzyme, the protonmotive Q cycle, is partly understood. The long term objectives of this research are to understand the mechanism of the cytochrome bc1 complex and to elucidate the pathway by which the subunits of this oligomeric enzyme complex are assembled into the inner mitochondrial membrane. The specific aims of the current research project are to answer the following questions regarding the mechanism. - Is ubiquinol oxidation a concerted or sequential reaction? - What is the structural basis of negative cooperativity in the binding of ubiquinol? - How are protons conducted to and from the ubiquinone and ubiquinol reaction sites? - How does ubisemiquinone stability affect the rate of ubiquinone reduction? We plan to investigate these questions in the cytochrome bc1 complex of the yeast Saccharomyces cerevisiae, since this enzyme can be modified by molecular genetics methods and isolated in quantities sufficient for biochemical and biophysical characterization.

细胞色素bc1复合体是一种参与细胞活动的能量转导酶