ANALYSIS AND CONTROL OF CHROMOSOME MOVEMENT

我们的目标是理解染色体的平均分布到 有丝分裂和减数分裂中的子细胞。对常态的一种解释 染色体分布的成功也有助于解释失败的原因 这会导致唐氏综合症和其他人类染色体疾病。 在有丝分裂中，均匀分布依赖于定向的染色体运动。 产生定向运动的有丝分裂力量将在 活细胞中的绝对单位。其目的是了解是什么导致了 染色体在前中期向赤道移动，在那里有丝分裂 电机的位置，并比较前中期和中期的力量生产 后期。微管在体外和细胞模型中将被 通过显微操作确定有丝分裂力是否会影响微管 结构以及微管组装是否产生力。 同一染色体在活细胞中和在细胞中的相关研究 提出了电子显微镜的概念。我们的目标是学习如何 产生均匀分布的染色体的微管排列出现 关于以及如何使张力稳定一个适当的安排 微管。

The goal is to understand the equal distribution of chromosomes to the daughter cells in mitosis and meiosis. An explanation for the normal success in chromosome distribution would also help explain the failures that result in Down's syndrome and other chromosome diseases in humans. Equal distribution depends upon directed chromosome movement in mitosis. The mitotic forces that produce directed movement would be measured in absolute units in living cells. The aims are to learn what causes chromosomes to move to the equator in prometaphase, where the mitotic motors are located, and to compare force production in prometaphase and anaphase. Microtubules in vitro and in a cell model will be micromanipulated to determine whether mitotic forces can affect microtubule structure and whether microtubule assembly generates force. Correlated studies of the same chromosome in living cells and in the electron microscope are proposed. The aims are to learn how the microtubule arrangement that produces equal chromosome distribution comes about and how tension forces stabilize the one appropriate arrangement of microtubules.