Proposal for a Fast Scanning Mass Spectrometer capable of Data Independent Acquisition for proteomics

关于能够独立采集蛋白质组数据的快速扫描质谱仪的提案

• 批准号: BB/T018089/1
• 负责人: Peter Ratcliffe
• 金额: $ 43.25万
• 依托单位: The Francis Crick Institute
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FT018089%2F1
• 关键词: Proposal; Fast; Scanning; Mass; Spectrometer

The Crick is a partnership between the Medical Research Council, Cancer Research UK, the Wellcome Trust and three leading universities: UCL (University College London), Imperial College London and King's College London. The Crick aspires to be one of the world's leading medical research institutes. The Crick achieves operational and research efficiencies and economies-of-scale through centralised facilities and functions, known as Science Operations, that provide all researchers at the Crick, irrespective of affiliation, with access to cutting-edge equipment, animals for research and laboratory enabling functions such as the Proteomics Science Technology Platform which houses cutting-edge mass spectrometry equipment. This technology can be used to investigate many aspects of proteins such as the quantification of protein expression, the discovery and characterisation of post-translational modifications and structural properties such as shape and solvent accessibility. This proposal is for a fast-scanning mass spectrometer capable of performing Data Independent Acquisition (DIA). The unprecedented scan speed, mass resolution and chromatographic stability that this system offers will enable studies that require high sensitivity, high throughput and high performance of label free peptide and protein quantification.

克里克是医学研究委员会、英国癌症研究中心、惠康信托基金会和三所顶尖大学（伦敦大学学院、伦敦帝国理工学院和伦敦国王学院）之间的合作伙伴关系。克里克医学院立志成为世界领先的医学研究机构之一。克里克通过被称为科学操作的集中设施和功能实现了运营和研究效率和规模经济，这为克里克的所有研究人员提供了最先进的设备，研究动物和实验室功能，如容纳尖端质谱设备的蛋白质组学科学技术平台。该技术可用于研究蛋白质的许多方面，如蛋白质表达的定量，翻译后修饰的发现和表征以及结构特性，如形状和溶剂可及性。该方案是一种能够进行数据独立采集（DIA）的快速扫描质谱仪。该系统提供的前所未有的扫描速度，质量分辨率和色谱稳定性将使需要高灵敏度，高通量和高性能无标签肽和蛋白质定量的研究成为可能。