Proposal for a Fast Scanning Mass Spectrometer capable of Data Independent Acquisition for proteomics

Crick是医学研究委员会、英国癌症研究中心、惠康信托基金和三所顶尖大学：伦敦大学学院、伦敦帝国理工学院和伦敦国王学院的合作伙伴。克里克立志成为世界领先的医学研究机构之一。克里克通过集中化的设施和功能实现运营和研究效率以及规模经济，这些设施和功能被称为科学运营，为克里克的所有研究人员提供访问尖端设备、用于研究的动物和实验室支持功能的机会，例如容纳尖端质谱学设备的蛋白质组学科学技术平台。这项技术可用于研究蛋白质的许多方面，如蛋白质表达的量化，翻译后修饰的发现和表征，以及形状和溶剂可及性等结构性质。这项建议是关于一种能够执行数据独立采集(DIA)的快速扫描质谱仪。该系统提供的前所未有的扫描速度、质量分辨率和层析稳定性将使需要无标记多肽和蛋白质定量的高灵敏度、高通量和高性能的研究成为可能。

The Crick is a partnership between the Medical Research Council, Cancer Research UK, the Wellcome Trust and three leading universities: UCL (University College London), Imperial College London and King's College London. The Crick aspires to be one of the world's leading medical research institutes. The Crick achieves operational and research efficiencies and economies-of-scale through centralised facilities and functions, known as Science Operations, that provide all researchers at the Crick, irrespective of affiliation, with access to cutting-edge equipment, animals for research and laboratory enabling functions such as the Proteomics Science Technology Platform which houses cutting-edge mass spectrometry equipment. This technology can be used to investigate many aspects of proteins such as the quantification of protein expression, the discovery and characterisation of post-translational modifications and structural properties such as shape and solvent accessibility. This proposal is for a fast-scanning mass spectrometer capable of performing Data Independent Acquisition (DIA). The unprecedented scan speed, mass resolution and chromatographic stability that this system offers will enable studies that require high sensitivity, high throughput and high performance of label free peptide and protein quantification.