Proposal for a Fast Scanning Mass Spectrometer capable of Data Independent Acquisition for proteomics

克里克是医学研究理事会、英国癌症研究中心、威康信托基金会和三所领先大学的合作伙伴关系：伦敦大学学院（伦敦）、伦敦帝国理工学院（伦敦）和伦敦国王学院（伦敦）。克里克立志成为世界领先的医学研究机构之一。克里克通过集中的设施和功能实现了运营和研究效率以及规模经济，称为科学运营，为克里克的所有研究人员提供了先进的设备，研究动物和实验室使能功能，如蛋白质组学科学技术平台，其中包含先进的质谱设备。该技术可用于研究蛋白质的许多方面，例如蛋白质表达的定量，翻译后修饰的发现和表征以及结构特性，例如形状和溶剂可及性。该建议是用于能够执行数据独立采集（DIA）的快速扫描质谱仪。该系统提供的前所未有的扫描速度、质量分辨率和色谱稳定性将使需要高灵敏度、高通量和高性能的无标记肽和蛋白质定量的研究成为可能。

The Crick is a partnership between the Medical Research Council, Cancer Research UK, the Wellcome Trust and three leading universities: UCL (University College London), Imperial College London and King's College London. The Crick aspires to be one of the world's leading medical research institutes. The Crick achieves operational and research efficiencies and economies-of-scale through centralised facilities and functions, known as Science Operations, that provide all researchers at the Crick, irrespective of affiliation, with access to cutting-edge equipment, animals for research and laboratory enabling functions such as the Proteomics Science Technology Platform which houses cutting-edge mass spectrometry equipment. This technology can be used to investigate many aspects of proteins such as the quantification of protein expression, the discovery and characterisation of post-translational modifications and structural properties such as shape and solvent accessibility. This proposal is for a fast-scanning mass spectrometer capable of performing Data Independent Acquisition (DIA). The unprecedented scan speed, mass resolution and chromatographic stability that this system offers will enable studies that require high sensitivity, high throughput and high performance of label free peptide and protein quantification.