Fast scanning two photon microscope with confocal unit for intravital and reflectance imaging
带有共焦装置的快速扫描双光子显微镜,用于活体和反射成像
基本信息
- 批准号:467868227
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:德国
- 项目类别:Major Research Instrumentation
- 财政年份:2021
- 资助国家:德国
- 起止时间:2020-12-31 至 无数据
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
The present proposal concerns a state of the art two photon microscope capable to record simultaneous multi-chromatic functional signals, such as calcium events, and perform high resolution subcellular morphological recordings on large fields of view in freely behaving animals.The requested equipment is central to the successful establishment of Dr. Nicolas Snaidero’s research group at the Hertie Institute for Clinical Brain research (HIH) and will in addition, provide technical solutions for many well establishes groups at the HIH and at the Center for Integrated Neuroscience (CIN) as part of the virtual imaging facility between these institutes. Indeed, currently the HIH and CIN have several two photon microscopes that are already heavily used and not suitable for the described projects due to major technical limitations. Indeed the innovative projects described in this application make this instrument absolutely necessary for the applicants and the entire institution.The Major scientific targets of the research groups listed in this proposal focus on the functional and morphological interaction between glial, epithelial, immune and neuronal cells in the brain and spinal cord but also in developing organoids and organs such as lung and liver. Advanced two photon imaging will be combined with complex models, behavioral studies and translational approaches to investigate axonal and glial pathologies as well as migration of immune cells in inflamed milieu, plaque dynamics and immune modulation in neurodegenerative disease models, neurotransmitter and hyperactivity on connectivity and brain development.To achieve these objectives a multi photon microscope equipped with two Ti:Sapphire lasers, one of which with an extended IR range up to 1300nm combined with fast resonant tandem scanner and high performance non descanned external detectors is required. The versatile nature of the requested system with adjustable excitation/detection allows optimal imaging of a wide variety of fluorophores and the adaptive stage is compatible with large behavioral setups. Furthermore, the integration of confocal components allows concomitant 2P and Spectral Confocal Reflectance Microscopy to combine label free imaging of myelin sheath with intravital imaging. Finally, the possibility for the system to be upgraded in order to perform 2P intravital Fluorescence Life time Imaging offers the unique opportunity to broaden dynamic applications especially towards metabolic state investigations and subcellular molecular changes.
目前的建议涉及到一种先进的双光子显微镜,能够同时记录多色功能信号,如钙事件,并在自由行为的动物的大视场上进行高分辨率的亚细胞形态学记录。所要求的设备是在Hertie临床脑研究所(HIH)成功建立Nicolas Snaidero博士研究小组的核心,此外,还将为HIH和综合神经科学中心(CIN)的许多建立良好的小组提供技术解决方案,作为这些研究所之间虚拟成像设施的一部分。事实上,目前HIH和CIN有几台双光子显微镜已经被大量使用,由于主要的技术限制,不适合上述项目。事实上,本申请中描述的创新项目使该工具对申请人和整个机构绝对必要。本提案中列出的研究小组的主要科学目标集中在脑和脊髓中胶质细胞、上皮细胞、免疫细胞和神经元细胞之间的功能和形态相互作用,以及发育中的类器官和器官,如肺和肝。先进的双光子成像将与复杂的模型、行为研究和转化方法相结合,研究炎症环境中轴突和神经胶质病理以及免疫细胞的迁移、神经退行性疾病模型中的斑块动力学和免疫调节、神经递质和多动症对连通性和大脑发育的影响。为了实现这些目标,需要配备两个Ti:Sapphire激光器的多光子显微镜,其中一个具有扩展到1300nm的红外范围,并结合快速谐振串联扫描仪和高性能非扫描外部探测器。所要求的系统具有可调节的激发/检测的通用性,允许各种荧光团的最佳成像,自适应阶段与大型行为设置兼容。此外,共聚焦组件的集成允许共聚焦显微镜和光谱共聚焦反射显微镜将髓鞘的无标记成像与活体成像结合起来。最后,系统升级的可能性,以执行2P活体荧光寿命成像提供了独特的机会,扩大动态应用,特别是在代谢状态研究和亚细胞分子变化。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
其他文献
Internet-administered, low-intensity cognitive behavioral therapy for parents of children treated for cancer: A feasibility trial (ENGAGE).
针对癌症儿童父母的互联网管理、低强度认知行为疗法:可行性试验 (ENGAGE)。
- DOI:
10.1002/cam4.5377 - 发表时间:
2023-03 - 期刊:
- 影响因子:4
- 作者:
- 通讯作者:
Differences in child and adolescent exposure to unhealthy food and beverage advertising on television in a self-regulatory environment.
在自我监管的环境中,儿童和青少年在电视上接触不健康食品和饮料广告的情况存在差异。
- DOI:
10.1186/s12889-023-15027-w - 发表时间:
2023-03-23 - 期刊:
- 影响因子:4.5
- 作者:
- 通讯作者:
The association between rheumatoid arthritis and reduced estimated cardiorespiratory fitness is mediated by physical symptoms and negative emotions: a cross-sectional study.
类风湿性关节炎与估计心肺健康降低之间的关联是由身体症状和负面情绪介导的:一项横断面研究。
- DOI:
10.1007/s10067-023-06584-x - 发表时间:
2023-07 - 期刊:
- 影响因子:3.4
- 作者:
- 通讯作者:
ElasticBLAST: accelerating sequence search via cloud computing.
ElasticBLAST:通过云计算加速序列搜索。
- DOI:
10.1186/s12859-023-05245-9 - 发表时间:
2023-03-26 - 期刊:
- 影响因子:3
- 作者:
- 通讯作者:
Amplified EQCM-D detection of extracellular vesicles using 2D gold nanostructured arrays fabricated by block copolymer self-assembly.
使用通过嵌段共聚物自组装制造的 2D 金纳米结构阵列放大 EQCM-D 检测细胞外囊泡。
- DOI:
10.1039/d2nh00424k - 发表时间:
2023-03-27 - 期刊:
- 影响因子:9.7
- 作者:
- 通讯作者:
的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('', 18)}}的其他基金
An implantable biosensor microsystem for real-time measurement of circulating biomarkers
用于实时测量循环生物标志物的植入式生物传感器微系统
- 批准号:
2901954 - 财政年份:2028
- 资助金额:
-- - 项目类别:
Studentship
Exploiting the polysaccharide breakdown capacity of the human gut microbiome to develop environmentally sustainable dishwashing solutions
利用人类肠道微生物群的多糖分解能力来开发环境可持续的洗碗解决方案
- 批准号:
2896097 - 财政年份:2027
- 资助金额:
-- - 项目类别:
Studentship
A Robot that Swims Through Granular Materials
可以在颗粒材料中游动的机器人
- 批准号:
2780268 - 财政年份:2027
- 资助金额:
-- - 项目类别:
Studentship
Likelihood and impact of severe space weather events on the resilience of nuclear power and safeguards monitoring.
严重空间天气事件对核电和保障监督的恢复力的可能性和影响。
- 批准号:
2908918 - 财政年份:2027
- 资助金额:
-- - 项目类别:
Studentship
Proton, alpha and gamma irradiation assisted stress corrosion cracking: understanding the fuel-stainless steel interface
质子、α 和 γ 辐照辅助应力腐蚀开裂:了解燃料-不锈钢界面
- 批准号:
2908693 - 财政年份:2027
- 资助金额:
-- - 项目类别:
Studentship
Field Assisted Sintering of Nuclear Fuel Simulants
核燃料模拟物的现场辅助烧结
- 批准号:
2908917 - 财政年份:2027
- 资助金额:
-- - 项目类别:
Studentship
Assessment of new fatigue capable titanium alloys for aerospace applications
评估用于航空航天应用的新型抗疲劳钛合金
- 批准号:
2879438 - 财政年份:2027
- 资助金额:
-- - 项目类别:
Studentship
Developing a 3D printed skin model using a Dextran - Collagen hydrogel to analyse the cellular and epigenetic effects of interleukin-17 inhibitors in
使用右旋糖酐-胶原蛋白水凝胶开发 3D 打印皮肤模型,以分析白细胞介素 17 抑制剂的细胞和表观遗传效应
- 批准号:
2890513 - 财政年份:2027
- 资助金额:
-- - 项目类别:
Studentship
Understanding the interplay between the gut microbiome, behavior and urbanisation in wild birds
了解野生鸟类肠道微生物组、行为和城市化之间的相互作用
- 批准号:
2876993 - 财政年份:2027
- 资助金额:
-- - 项目类别:
Studentship
相似国自然基金
基于太赫兹光谱近场成像技术的应力场测量方法
- 批准号:11572217
- 批准年份:2015
- 资助金额:120.0 万元
- 项目类别:面上项目
红树对重金属的定位累积及耦合微观分析与耐受策略研究
- 批准号:30970527
- 批准年份:2009
- 资助金额:35.0 万元
- 项目类别:面上项目
化石硅藻微构造与古环境和古气候研究
- 批准号:40442004
- 批准年份:2004
- 资助金额:10.0 万元
- 项目类别:专项基金项目
相似海外基金
Fast Multi-Functional 3D Imaging of Cellular Activities in Deep Tissue
深层组织细胞活动的快速多功能 3D 成像
- 批准号:
10861526 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Fast motion-robust fetal neuroimaging with MRI
使用 MRI 进行快速运动稳健的胎儿神经成像
- 批准号:
10545512 - 财政年份:2022
- 资助金额:
-- - 项目类别:
Deep and fast imaging using adaptive excitation sources
使用自适应激励源进行深度快速成像
- 批准号:
10516870 - 财政年份:2022
- 资助金额:
-- - 项目类别:
Fast, large area, multiphoton exoscope (FLAME) for improving early detection of melanoma
快速、大面积、多光子外窥镜 (FLAME) 用于改善黑色素瘤的早期检测
- 批准号:
10687990 - 财政年份:2022
- 资助金额:
-- - 项目类别:
Fast, large area, multiphoton exoscope (FLAME) for improving early detection of melanoma
快速、大面积、多光子外窥镜 (FLAME) 用于改善黑色素瘤的早期检测
- 批准号:
10365803 - 财政年份:2022
- 资助金额:
-- - 项目类别:
Fast volumetric imaging of oxygen delivery in the mouse brain at single red blood cell resolution
以单红细胞分辨率对小鼠大脑中的氧气输送进行快速体积成像
- 批准号:
10525881 - 财政年份:2022
- 资助金额:
-- - 项目类别:
Development of 3D-FAST Optical Interface for Rapid Volumetric Neural Sensing and Modulation
开发用于快速体积神经传感和调制的 3D-FAST 光学接口
- 批准号:
10294019 - 财政年份:2021
- 资助金额:
-- - 项目类别:
Fast motion-robust fetal neuroimaging with MRI
使用 MRI 进行快速运动稳健的胎儿神经成像
- 批准号:
10756678 - 财政年份:2020
- 资助金额:
-- - 项目类别:
Development of Line-Scan Temporal Focusing for fast structural imaging of synapse assembly/disassembly in vivo
开发线扫描时间聚焦,用于体内突触组装/拆卸的快速结构成像
- 批准号:
10301729 - 财政年份:2020
- 资助金额:
-- - 项目类别:
Fast motion-robust fetal neuroimaging with MRI
使用 MRI 进行快速运动稳健的胎儿神经成像
- 批准号:
10197182 - 财政年份:2020
- 资助金额:
-- - 项目类别: