ISOLATION OF VISUAL PATHWAY MUTANTS IN THE FISH

鱼视觉通路突变体的分离

• 批准号: 3265719
• 负责人: WALTER GILBERT
• 金额: $ 39.97万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-01-01 至 1994-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3265719
• 关键词: alternatives to animals in research; aquatic organism; autosomal recessive trait; biological models; embryo /fetus; fish; fresh water environment; gene expression; genetic regulatory element; genome; model design /development; molecular cloning; molecular genetics; mutant; neuroanatomy; neurogenesis; neurophysiology; nontherapeutic iontophoresis; parthenogenesis; reporter genes; tissue /cell culture; transposon /insertion element; vision disorders; visual pathways

This project seeks to understand the development of the vertebrate visual system using genetic analysis. By producing mutations via insertional DNA techniques we will be able to identify genes responsible for normal visual development in a vertebrate. We will insert DNA into the germline of fish species that: (1) have a short generation time; (2) can be scored rapidly for recessive mutations by artificial parthenogenesis; (3) can be cloned; and (4) are easily scored for visual defects. Some of the insertions will cause changes in the normal development of the visual system. Some of these changes should provide basic insights into this complex process. We will use anatomy, physiology, and biochemistry to analyze these mutants. We will apply a host of modern molecular biological techniques to identify and characterize the genes, the function of which is known from their phenotypes.

这个项目旨在了解脊椎动物视觉的发展 系统进行遗传分析。通过插入DNA产生突变 技术，我们将能够识别基因负责正常的视觉 脊椎动物的发育。 我们将把DNA插入到鱼类的生殖系中，这些鱼类：（1）具有 世代时间短;（2）可快速对隐性突变进行评分 通过人工孤雌生殖;（3）可以克隆;（4）容易 视觉缺陷评分。一些插入会导致 视觉系统的正常发育。其中一些变化应 为这个复杂的过程提供了基本的见解。 我们将使用解剖学、生理学和生物化学来分析这些 变种人我们将运用大量现代分子生物学技术， 识别和表征基因，其功能已知于 它们的表型。