MOLECULAR BIOLOGY OF INTERCELLULAR COMMUNICATION

拟议的研究将研究缝隙连接的结构和功能 作为细胞间通讯的场所。连接结构将是 利用X射线衍射、电子显微镜和电子显微镜技术进行了研究 生物化学。将对心脏、肝脏和晶状体组织的缝隙连接进行比较。 将通过蛋白质分解片段的肽图进行比较 不同缝隙连接蛋白中主要多肽的电泳分离 准备工作。缝隙连接的功能和生物发生将通过 制备抗晶状体缝隙连接主肽的单抗。这个 抗体将用于研究缝隙连接的细胞和分子生物学 生物合成和组装，寻找晶状体中mRNA的初级转录本 匀浆和带有荧光抗体的结合外抗原。冰冻 替代放射自显影将用于研究新陈代谢的合作 器官水平。

The proposed research will study the structure and function of the gap junction as the site of intercellular communication. The junction structure will be studied using techniques of x-ray diffraction, electron microscopy, and biochemistry. Gap junctions from heart, liver and lens tissue will be compared. Comparison will be made by peptide mapping of proteolysis fragments of principal polypeptides electrophoretically isolated from different gap junction preparations. The function and biogenesis of gap junctions will be studied by raising monoclonal antibodies to the lens gap junction principal peptide. The antibodies will be used to study the cell and molecular biology of gap junction biosynthesis and assembly, looking for primary transcripts of mRNA from lens homogenates, and extrajunctional antigens with fluorescent antibodies. Freeze substitution autoradiography will be used to study metabolic cooperation at the organ level.