RNA SYNTHESIS BY BACTERIAL RNA POLYMERASE
RNA SYNTHESIS BY BACTERIAL RNA POLYMERASE
批准号:
3269347
负责人:
JOSEPH S KRAKOW
金额:
$15.8万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1978
资助国家:
美国
项目状态:
已结题
起止时间:
1978-11-01 至 1990-03-31
关键词:
Azotobacter vinelandii DNA binding protein DNA directed RNA polymerase Escherichia coli antibody formation bacterial RNA chemical models conformation cyclic AMP receptors enzyme induction /repression enzyme linked immunosorbent assay enzyme mechanism enzyme structure gel electrophoresis genetic library genetic mapping high performance liquid chromatography laboratory mouse molecular site monoclonal antibody nucleic acid reconstitution nucleic acid sequence protein sequence radiotracer tritium
中文摘要
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英文摘要
The purpose of the proposed research is to gain further information on the
relation of structure to the functional properties of RNA polymerase using
for the most part an immunological approach. We will generate a library of
monoclonal antibodies raised against determinants on the separate subunits
and the intact RNA polymerase. It is hoped that such a collection would
contain monoclonal antibodies which were sequence and/or conformation
dependent with regard to antigenic determinants on RNA polymerase.
Following chemical and enzymatic cleavage the sites of the antigenic
determinants will be determined where possible by limited N-terminal
sequencing of the polypeptide fragments. Mapping of the antigenic domains
will indicate which areas of the polypeptide chains in the native folded
structure are on the surface of the free and polymerase-associated
subunits. Correlation of the (putative) effects of monoclonal antibodies
on subunit-subunit assembly, sigma, rho and cAMP receptor protein mediated
effects, inhibition of steps in the polymerase reaction, and possible
protective effects with temperature-sensitive polymerase mutants with the
antigenic domains can relate enzyme structure with function. The probable
conformational changes in RNA polymerase during reconstitution from
subunits, promoter binding and formation of the ternary elongation
complexes can be studied using those monoclonal antibodies whose antigenic
determinants are affected by such complexes. The phylogenetic relationship
among the antigenic determinants of RNA polymerase from different bacterial
species will also be investigated. Studies on the effect of chemical
modification of the alpha subunit on reconstitution and polymerase activity
will be continued.
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STUDIES ON THE CYCLIC AMP RECEPTOR PROTEIN OF E COLI
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批准号:6240186
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项目类别:
-
资助金额:$2.6万
-
财政年份:1997
-
负责人:JOSEPH S KRAKOW
-
依托单位:
SMALL INSTRUMENTATION GRANT
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批准号:3523711
-
项目类别:
-
资助金额:$3.61万
-
财政年份:1993
-
负责人:JOSEPH S KRAKOW
-
依托单位:
DIODE ARRAY SPECTROPHOTOMETER, HPLC, DATA PROCESSING
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批准号:3523180
-
项目类别:
-
资助金额:$2.27万
-
财政年份:1987
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负责人:JOSEPH S KRAKOW
-
依托单位:
BIOMEDICAL RESEARCH SUPPORT
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批准号:3518393
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项目类别:
-
资助金额:$8.19万
-
财政年份:1986
-
负责人:JOSEPH S KRAKOW
-
依托单位:
BIOMEDICAL RESEARCH SUPPORT
-
批准号:3518392
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项目类别:
-
资助金额:$7.76万
-
财政年份:1985
-
负责人:JOSEPH S KRAKOW
-
依托单位:
BIOMEDICAL RESEARCH SUPPORT
-
批准号:3518396
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项目类别:
-
资助金额:$6.49万
-
财政年份:1979
-
负责人:JOSEPH S KRAKOW
-
依托单位:
RNA SYNTHESIS BY BACTERIAL RNA POLYMERASE
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批准号:3269346
-
项目类别:
-
资助金额:$16.01万
-
财政年份:1978
-
负责人:JOSEPH S KRAKOW
-
依托单位:
RNA SYNTHESIS BY BACTERIAL RNA POLYMERASE
-
批准号:3269342
-
项目类别:
-
资助金额:$15.34万
-
财政年份:1978
-
负责人:JOSEPH S KRAKOW
-
依托单位:
RNA SYNTHESIS BY BACTERIAL RNA POLYMERASE
-
批准号:3269345
-
项目类别:
-
资助金额:$14.9万
-
财政年份:1978
-
负责人:JOSEPH S KRAKOW
-
依托单位:
RNA SYNTHESIS BY BACTERIAL RNA POLYMERASE
-
批准号:3269348
-
项目类别:
-
资助金额:$16.73万
-
财政年份:1978
-
负责人:JOSEPH S KRAKOW
-
依托单位:
STUDIES ON THE CYCLIC AMP RECEPTOR PROTEIN OF E. COLI
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批准号:3271229
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项目类别:
-
资助金额:$13.88万
-
财政年份:1976
-
负责人:JOSEPH S KRAKOW
-
依托单位:
STUDIES ON THE CYCLIC AMP RECEPTOR PROTEIN OF E. COLI
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批准号:3271223
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项目类别:
-
资助金额:$12.25万
-
财政年份:1976
-
负责人:JOSEPH S KRAKOW
-
依托单位:
STUDIES ON THE CYCLIC AMP RECEPTOR PROTEIN OF E COLI
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批准号:2173953
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项目类别:
-
资助金额:$15.21万
-
财政年份:1976
-
负责人:JOSEPH S KRAKOW
-
依托单位:
STUDIES ON THE CYCLIC AMP RECEPTOR PROTEIN OF E COLI
-
批准号:3271225
-
项目类别:
-
资助金额:$15.47万
-
财政年份:1976
-
负责人:JOSEPH S KRAKOW
-
依托单位:
STUDIES ON THE CYCLIC AMP RECEPTOR PROTEIN OF E COLI
-
批准号:3271222
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项目类别:
-
资助金额:$12.58万
-
财政年份:1976
-
负责人:JOSEPH S KRAKOW
-
依托单位:
THE CYCLIC AMP RECEPTOR PROTEIN OF E COLI
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批准号:3271230
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项目类别:
-
资助金额:$14.43万
-
财政年份:1976
-
负责人:JOSEPH S KRAKOW
-
依托单位:
STUDIES ON THE CYCLIC AMP RECEPTOR PROTEIN OF E. COLI
-
批准号:3271227
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项目类别:
-
资助金额:$13.49万
-
财政年份:1976
-
负责人:JOSEPH S KRAKOW
-
依托单位:
STUDIES ON THE CYCLIC AMP RECEPTOR PROTEIN OF E. COLI
-
批准号:3271228
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项目类别:
-
资助金额:$13.14万
-
财政年份:1976
-
负责人:JOSEPH S KRAKOW
-
依托单位:
CYCLIC AMP RECEPTOR PROTEIN AND RNA SYNTHESIS BY RNA POLYMERASE
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批准号:4705440
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:JOSEPH S KRAKOW
-
依托单位:
STUDIES ON THE CYCLIC AMP RECEPTOR PROTEIN OF E COLI
-
批准号:5211804
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项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:JOSEPH S KRAKOW
-
依托单位:--
海外基金