LEUKOCYTE FUNCTION IN THE PATHOLOGY OF BURN INJURY

这项拟议的研究旨在澄清 导致异常的可识别的生化异常 中性粒细胞功能、创伤事件和全身 在热损伤患者中看到的变化。这些研究将集中在： 1)确定氧化功能降低的机制和状态 在完整的白细胞中的活性氧物种；2)与这些有关 对烧伤血清/血浆或体液中因子的氧化反应；3) 影响血清和体液因子(S)的分离和鉴定 氧化过程，从而杀死细菌；4)将 主要表现为功能性氧合异常和结构改变 电子显微镜和组织化学测定以表征 循环和伤口液细胞群；以及5)试图联系 创面环境(细胞和液体)观察的类型 在烧伤伤口上定植的微生物。对生化事件的理解 中性粒细胞的功能及其与细菌定植的关系是必不可少的 在适当的局部或系统治疗干预之前 深思熟虑。

The proposed research is designed to clarify the relationship between identifiable biochemical aberrations responsible for abnormal polymorphonuclear leukocyte function, the wound events, and systemic changes seen in thermally injured patients. The studies will be focused: 1) to identify mechanisms of decreased oxidative functions and the status of reactive oxygen species in intact leukocytes; 2) to relate these oxidative responses to factors in burn serum/plasma or would fluid; 3) to isolate and characterize the sera and fluid factor(s) which affect oxidative processes and therefore bacterial kill; 4) to correlate the domonstrated functional O2 abnormalities with structural changes by electron microscopy and histochemical determinations to characterize circulating and wound fluid cell populations; and 5) to attempt to relate wound environment (cellular and fluid) observations to the types of organisms colonizing burn wounds. Understanding of the biochemical events in PMN function and their relation to bacterial colonization is essential before appropriate local or systemic therapeutic interventions can be contemplated.