MICROTUBULES AND AXOPLASM IN OLFACTORY NEURONS

嗅觉神经元中的微管和轴浆

• 批准号: 3272714
• 负责人: PAUL R BURTON
• 金额: $ 7.81万
• 依托单位: UNIVERSITY OF KANSAS LAWRENCE
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-06-01 至 1987-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3272714
• 关键词: autoradiography; axon; cell cycle; cell differentiation; cell membrane; electron microscopy; histochemistry /cytochemistry; histopathology; host organism interaction; neuroanatomy; neuroblastoma; organelles; radiotracer; scanning electron microscopy; sperm; spermicide

This research focuses on cytoskeletal elements of frog olfactory axons, with emphasis on microtubules. The three-dimensional relationships between axoplasmic microtubules and neutrofiliments will be studied with the electron microscope. Cytochemical tests will be used on the ultrastructural level to localize ATPase activity in olfactory axons, and since some axons contain only neurofilaments and no microtubules, it will be of interest to see if these show ATPase activity distributed in the same manner as in axons containing both neurofilaments and microtubules. The substructure of isolated neurofilaments and neurofilaments in situ will be studied. Homogenates of olfactory nerve will be examined by SDS disc and slab gel electrophoresis to characterize the kinds of proteins present, particularly cytoskeletal proteins. Studies of the polarity of axonal microtubules will be continued, and these will involve the addition of soluble tubulin to preexisting microtubules as ribbon elements whose cross-sectional curvature indicates the polarity of the microtubules to which they are attached (ribbons either curve clockwise or counterclockwise). Finally, tracer studies will be used to test the hypothesis that bidirectional transport occurs in olfactory nerve axons, and these findings will be related to evidence regarding microtubule polarity.

本研究的重点是青蛙嗅觉轴突的细胞骨架元素， 重点是微管。 三维关系， 轴浆微管和纤维将研究与电子 显微镜 细胞化学检测将用于超微结构水平， ATP酶定位于嗅觉轴突中的活性，并且由于一些轴突仅含有 神经丝和没有微管，这将是有趣的，看看这些显示， ATP酶活性的分布方式与含有两者的轴突相同。 神经丝和微管。 分离的神经丝的亚结构 和神经丝的研究。 嗅神经的匀浆 采用SDS圆盘和平板凝胶电泳法对不同的菌株进行鉴定 尤其是细胞骨架蛋白。 的研究 轴突微管的极性将继续，这些将涉及 将可溶性微管蛋白添加到预先存在的微管中作为带状元件， 横截面曲率表明了微管的极性， 它们被连接（带顺时针或逆时针弯曲）。 最后，示踪剂研究将被用来测试假设，双向 运输发生在嗅神经轴突，这些发现将与 关于微管极性的证据。