MICROTUBULES AND AXOPLASM IN OLFACTORY NEURONS

这项研究集中在青蛙嗅觉轴突的细胞骨架元素上， 强调微管。两国之间的三维关系 轴浆微管和中性微管将用电子进行研究 显微镜。将在超微结构水平上使用细胞化学测试来 定位嗅觉轴突中的ATPase活性，因为一些轴突只包含 神经丝和没有微管，这将是有趣的，看看这些显示 ATPase活性在轴突中的分布方式与包含两者的轴突相同 神经丝和微管。分离的神经细丝的亚结构 以及神经细丝的原位研究。嗅神经匀浆 用十二烷基硫酸钠纸片和平板凝胶电泳法进行鉴定 存在的蛋白质，特别是细胞骨架蛋白质。对中国传统文化的研究 轴突微管的极性将继续，这些将涉及 将可溶性微管蛋白添加到先前存在的微管中作为带状元件，其 横截面曲率表示微管的极性 它们连接在一起(丝带顺时针或逆时针弯曲)。 最后，将使用示踪剂研究来检验双向 嗅神经轴突发生运输，这些发现将与 关于微管极性的证据。

This research focuses on cytoskeletal elements of frog olfactory axons, with emphasis on microtubules. The three-dimensional relationships between axoplasmic microtubules and neutrofiliments will be studied with the electron microscope. Cytochemical tests will be used on the ultrastructural level to localize ATPase activity in olfactory axons, and since some axons contain only neurofilaments and no microtubules, it will be of interest to see if these show ATPase activity distributed in the same manner as in axons containing both neurofilaments and microtubules. The substructure of isolated neurofilaments and neurofilaments in situ will be studied. Homogenates of olfactory nerve will be examined by SDS disc and slab gel electrophoresis to characterize the kinds of proteins present, particularly cytoskeletal proteins. Studies of the polarity of axonal microtubules will be continued, and these will involve the addition of soluble tubulin to preexisting microtubules as ribbon elements whose cross-sectional curvature indicates the polarity of the microtubules to which they are attached (ribbons either curve clockwise or counterclockwise). Finally, tracer studies will be used to test the hypothesis that bidirectional transport occurs in olfactory nerve axons, and these findings will be related to evidence regarding microtubule polarity.