BIOLOGICAL ELECTRON TRANSFER

生物电子转移

• 批准号: 3270379
• 负责人: MICHAEL Anthony CUSANOVICH
• 金额: $ 10.48万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-03-01 至 1987-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3270379
• 关键词: Pseudomonas aeruginosa; Rhodospirillum; bioenergetics; chemical reaction; chemical structure function; cytochrome c; electron transport; hemoprotein structure; hydrogen transport; ions; iron cyanide; metalloproteins; molecular site; myocardium; oxidation reduction reaction; photosynthesis; saltwater environment; stop flow technique; sulfur metabolizing bacteria

Past studies on the mechanism of electron transfer by c-type cytochromes have yielded considerable information and have led us to formulate a number of interesting and potentially significant questions, including: the role of the protein surface structure and protein dynamics in electron transfer. The ultimate goal of this work remains to understand the mechanism of coupling biological electron transfer to energy conservation. However, to fully understand this process a number of related issues must be addressed which bear on both biological electron transfer and the general areas of prot ein structure and function. Thus, an integral part of elucidating the mechanism of biological electron transfer focuses on the chemical and structural aspects of the interaction between two proteins which give these reactions their high specificity and rapid rates. The studies proposed here are designed to address both the question of the mechanism of biological electron transport and the nature of protein-protein interactions. Specifically we propose to investigate the inter-actions of cytochrone c with non-heme iron sulfur proteins and photosynthetic reaction centers. We also are proposing experiments designed to elucidate the surface structure and dynamics of cytochromes in solution and to use this information in understanding protein-protein interactions and electron transfer. We anticipate that information derived from these studies will bear not only the mechanism of biological electron transfer but will provide information useful in understanding the general role of dynamics in protein function and the nature of specific chemical and physical inter-actions in reactions involving the interaction of macromolecules.

C型细胞色素电子传递机理的研究进展 已经产生了大量的信息，并导致我们制定了一些 一些有趣和潜在的重要问题，包括： 蛋白质的表面结构和蛋白质的电子动力学 转移 这项工作的最终目标仍然是了解 生物电子传递与能量守恒耦合的机制。 然而，要充分理解这一过程，必须了解一些相关问题， 这两个生物电子转移和 蛋白质结构和功能的一般领域。 因此， 阐明生物电子转移机制的重点是 两种蛋白质之间相互作用的化学和结构方面 这使这些反应具有高特异性和快速性。 的 这里提出的研究旨在解决这两个问题， 生物电子传递的机制和 蛋白质相互作用 具体来说，我们建议调查 细胞色素c与非血红素铁硫蛋白的相互作用， 光合反应中心 我们还提议进行实验 旨在阐明细胞色素的表面结构和动力学， 并利用这些信息来理解蛋白质-蛋白质 相互作用和电子转移。 我们预计， 从这些研究将承担不仅机制的生物电子 转移，但将提供有用的信息，了解一般 动力学在蛋白质功能中的作用和特定化学物质的性质 以及反应中的物理相互作用， 大分子