DEVELOPMENTAL GENETICS WITH AN INDUCIBLE GENE SYSTEM

乙醇脱氢酶-1（Adhl）基因是10种主要多肽（ANP）之一， 在无氧压力下合成的。 该系统可供 重组DNA技术就像动物中的热休克系统一样。 的 最重要的目标是了解每一个 高等生物中“典型”基因的组成部分以及基因如何协调 在发育过程中数量和器官特异性。 我们将继续诱导，通过花粉选择恢复，并分析几个 Adhl突变体在调节参数如时间，速率， 器官特异性、稳定性或表达回路。 或者会利用有趣 突变体，包括不稳定的“控制元件”等位基因，我们将发现 该步骤是基因表达--例如，转录，RNA加工， 翻译--负责监管行为。 在其他测试中， 定量北方杂交技术和遗传精细结构 将使用分析。 我们还将表征蛋白质水平的分子 10种主要ANP和多肽的生物学特性， 好氧到厌氧环境（TP）; ANP的酶功能， 将寻求TP。 此外，我们计划开始一个正式的、定量的 遗传学研究将厌氧基因作为一个共同适应的复合体， 继续开发以Adhl为载体的原生质体转化系统。 选择标记

The alcohol dehydrogenase-l (Adhl) gene is one of 10 major polypeptides (ANPs) synthesized in response to anaerobic stress. The system is accessible to recombinant DNA technology much as is the heat-shock system in animals. The overriding objective is to understand the behavior and structure of every component of a "typical" gene in higher organisms and how genes are coordinated quantitatively and organ specifically during development. We will continue to induce, recover via pollen selection, and analyze several Adhl mutants altered in regulatory parameters such as timing, rate, organ-specificity, stability, or circuitry of expression. Using interesting mutants, including unstable "controlling element" alleles, we will discover which step is gene expression -- e.g., tranascription, RNA-processing, translation -- is responsible for the regulatory behavior. Among other tests, quantitative Northern hybridization techniques and genetic fine structure analyses will be used. We will also characterize the protein-level molecular biology of the 10 major ANPs and polypeptides specific to the transition from an aerobic to an anaerobic environment (TPs); enzymatic functions for the ANPs and TPs will be sought. In addition, we plan to begin a formal, quantitative genetic study treating the anaerobic genes as a co-adapted complex, and to continue development of a protoplast transformation system using Adhl as the selectable marker.