BIOCHEMICAL STUDIES OF E. COLI EXONUCLEASE V11

大肠杆菌核酸外切酶 V11 的生化研究

• 批准号: 3271641
• 负责人: JOHN W CHASE
• 金额: $ 14.07万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-01-01 至 1986-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3271641
• 关键词: DNA directed DNA polymerase; DNA repair; Escherichia coli; analytical method; bacterial virus; chemical kinetics; enzyme induction /repression; enzyme structure; enzyme substrate complex; exonuclease; genetic manipulation; mutant; reagent /indicator; ultraviolet radiation

The goals of this research program are to further elucidate the in vivo and in vitro functions of E. coli exonuclease VII and single-strand DNA binding protein. We propose to study the cellular control of the production of exonuclease VII. It is hoped that this information will allow us to isolate a strain of E. coli which produces increased amounts of the enzyme. Such a strain will serve as a general source of exonuclease VII both for our own in vitro studies as well as a source to others. The elucidation of the cellular control of the production of exonuclease VII may serve as a general model for the control of other nucleic acid enzymes, e.g., DNA polymerase III. For this reason we propose to study the cellular control of the production of DNA polymerase III. We will purify exonuclease VII to homogeneity, determine its subunit structure and initiate further in vitro biochemical studies of the homogeneous enzyme. We will extend our studies of the role of exonuclease VII in DNA repair. Finally, the amino acid sequence of E. coli single-strand binding protein from wild type and mutant strains will be determined. This will aid in elucidating the structure-function relationship between DNA binding protein and nucleic acids.

本研究计划的目标是进一步阐明在体内和体内

项目成果

DNA repair in E. coli strains deficient in single-strand DNA binding protein.

缺乏单链 DNA 结合蛋白的大肠杆菌菌株中的 DNA 修复。

• DOI: 10.1007/bf00270638
• 发表时间: 1981
• 期刊: Molecular & general genetics : MGG
• 作者: Whittier,RF;Chase,JW
• 通讯作者: Chase,JW