课题基金 / 基金详情

IN VITRO ANALYSIS OF A DNA REPLICATION APPARATUS

IN VITRO ANALYSIS OF A DNA REPLICATION APPARATUS
DNA 复制装置的体外分析
批准号:
3272267
负责人:
NAVIN K SINHA
金额:
$12.19万
依托单位国家:
美国
项目类别:
财政年份:
1977
资助国家:
美国
项目状态:
已结题
起止时间:
1977-08-01 至 1986-07-31

项目摘要

项目成果

NAVIN K SINHA的其他基金

相似基金

相关文献

中文摘要
翻译
计划使用纯化的T4噬菌体复制蛋白
英文摘要
It is planned to use purified replication proteins of bacteriophage T4 to analyze the mechanisms involved in generation of high fidelity during DNA replication. The accuracy of T4 DNA polymerase (in the absence of assessory proteins) during the duplication of natural DNA templates will be measured in a number of different DNA sequence contexts. The influence of a variety of different factors, with special emphasis on polymerase accessory proteins, on the accuracy of this reaction will be measured. The role of these proteins will also be investigated by substituting mutant species in DNA replication reactions catalyzed by a seven enzyme replication complex. Attempts will be made to analyze the relative contributions of base mispairing, base stacking, efficiency of proofreading and longer range DNA sequence factors on the accuracy of DNA replication. An in vitro system for the study of frameshift mutations will be established so that the mechanisms involved in their generation can be analyzed in great detail.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
IN VITRO ANALYSIS OF A DNA REPLICATION APPARATUS
  • 批准号:
    3272265
  • 项目类别:
  • 资助金额:
    $14.84万
  • 财政年份:
    1977
  • 负责人:
    NAVIN K SINHA
  • 依托单位:
IN VITRO ANALYSIS OF A DNA REPLICATION APPARATUS
  • 批准号:
    3272268
  • 项目类别:
  • 资助金额:
    $17.08万
  • 财政年份:
    1977
  • 负责人:
    NAVIN K SINHA
  • 依托单位:
IN VITRO ANALYSIS OF A DNA REPLICATION APPARATUS
  • 批准号:
    3272269
  • 项目类别:
  • 资助金额:
    $16.44万
  • 财政年份:
    1977
  • 负责人:
    NAVIN K SINHA
  • 依托单位:
海外基金