GENETIC STABILITY OF MOUSE ZYGOTES IN FROZEN STORAGE

低温保护剂：二甲基亚砜，甘油， 乙二醇以及冻融对基因突变率的影响 在八细胞小鼠胚胎中进行研究。 BALB/cBy胚胎 将在体外用低温保护剂处理近交系， 再植入或在液氮中冷冻并解冻然后再植入。 的 当成熟时，将活产小鼠与相同品系的未处理小鼠交配。 后代将与处理的亲本杂交以获得试验小鼠， 如果是纯合的，可能会显示隐性突变 为了优化 许多基因很容易被筛选出突变，两大组基因已经被 选择：确定组织相容性抗原的集合（通过 尾皮移植交换）和决定形态发生的集合（由 骨测量的多变量分析）。

The effects of the cryoprotective agents: dimethyl sulfoxide, glycerol, ethylene glycol, as well as of freezing and thawing on the rate of gene mutation in the eight-cell mouse embryo will be investigated. Embryos of the BALB/cBy inbred strain will be treated in vitro with a cryoprotective agent and reimplanted or frozen in liquid nitrogen and thawed and then reimplanted. The liveborn, when mature, will be mated to an untreated mouse of the same strain. The progeny will be crossed back to the treated parent to obtain test mice, which if homozygous, could reveal recessive mutations. In order to optimize the number of genes easily screened for mutation, two large sets of genes have been chosen: the set that determines histocompatibility antigens (screened by tailskin graft exchanges) and the set that determines morphogenesis (screened by multivariate analysis of bone measurements).