NORMAL VARIANT GENES OF MORPHOGENESIS

由B6衍生的25只B6.C同源品系小鼠 (C57BL/6BY)和BALB/CBY近交系的分离 组织相容性基因，将被用来识别影响 骨骼形态上的微小变化。来自同源基因的骨骼 菌株和来自B6的背景菌株将由木瓜酶制备 消化，骨骼将在显微镜下测量，获得50米 特征，数据将被简化为一组不相关的变量 (多维(超)空间的轴由正则变量分析 合并的分散矩阵)。显著的差异(距离在 衍生超空间)在同源菌株和背景菌株之间 将标志着感兴趣基因的存在。它的方向是 同源菌株位于远离背景菌株的地方 超空间将独一无二地定义基因效应。无论是 每个同源菌株最初所属的组织相容基因 构建的基因或另一个基因是具有形态发生效应的基因， 将通过将每个指定的同源菌株回交到 背景压力。子代的皮肤移植测试将揭示 维持与组织相容基因的关联性，从而 为组织相容性基因的多效性效应提供证据 形态学。将在指定的同源菌株与B6之间进行杂交 揭示基因表达的显性-隐性和上位性。

A set of 25 B6.C congenic strains of mice, which were derived from B6 (C57BL/6By) and BALB/cBy inbred strains for the purpose of isolating histocompatibility genes, will be used to identify the genes that effect small changes in morphology of the skeleton. Skeletons from the congenic strains and from the B6 background strain will be prepared by papain digestion, bones will be measured under a microscope to obtain 50 metric traits, and the data will be reduced to a set of uncorrelated variates (axes of a multidimensional (hyper) space by canonical variate analysis of pooled dispersion matrices). A significant difference (distance in the derived hyperspace) between a congenic strain and the background strain will signify the presence of a gene of interest. The direction in which the congenic strain is located away from the background strain in the hyperspace will uniquely define the gene effect. Whether the histocompatibility gene for which each congenic strain was originally constructed, or another gene, is the one with the morphogenetic effect, will be tested by backcrossing each indicated congenic strain to the background strain. Skin graft tests in the progeny will reveal whether association with the histocompatibility gene is maintained, thereby providing evidence on pleiotropic effects on histocompatibility genes on morphology. Crosses between indicated congenic strains and B6 will be made to reveal the dominance-recessivity and epistatic nature of gene expression.