FLAVOPROTEINS IN FATTY ACID METABOLISM

这项研究的总体目标是更深入地了解 酰辅酶A脱氢酶的结构、作用机制及代谢调控。 猪肾酰辅酶A脱氢酶的碘乙酸钝化衍生物 酸和几种二酮试剂正在调查中，以确定 催化必需氨基酸残基。两种基于作用机制的抑制剂 该酶亚甲基丙基乙酰-辅酶A和3，4-戊二烯酰-辅酶A在 研究和初步结果提示底物的一种机制。 涉及碳负离子形成的脱氢反应。 酰基辅酶A对红色、阴离子、倍喹酮的稳定作用机理 衍生品正在接受调查。这一结果应该有助于 解释这些酰基辅酶A脱氢酶的作用机制。 我们将扩大我们对猪肾脏重建的初步研究 使用环修饰黄素类似物的通用酰辅酶A脱氢酶。 将继续努力制定令人满意的猪只净化方案 肾脏电子转移黄素蛋白。

The overall objective of this research is to gain greater insight into the structure, mechanism of action and metabolic control of acyl-CoA dehydrogenases. Inactive derivatives of the pig kidney acyl-CoA dehydrogenase with iodoacetic acid and several dione reagents are under investigation to identify catalytically essential amino acid residues. Two mechanism based inhibitors of the enzyme, methylenecylcopropylacetyl-CoA and 3,4-pentadienoyl-CoA, are under investigation and preliminary results are suggestive of a mechanism of substrate dehydrogenation involving carbanion formation. The mechanism of stabilization of the red, anionic, seiquinone by acyl-CoA derivatives is under investigation. The results should aid in the interpretation of the mechanism of action of these acyl-CoA dehydrogenases. We will extend our preliminary studies on the reconstitution of pig kidney general acyl-CoA dehydrogenase using ring modified flavin analogs. Efforts will continue to develop a satisfactory purification scheme for pig kidney electron transferring flavoprotein.