MECHANISM OF PROTEIN SYNTHESIS INTITIATION

蛋白质合成起始机制

• 批准号: 3274246
• 负责人: WILLIAM C. MERRICK
• 金额: $ 16.7万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-07-01 至 1988-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3274246
• 关键词: RNA methylation; adenosine triphosphate; aminoacid analyzer; density gradient ultracentrifugation; eukaryote; gel electrophoresis; messenger RNA; radiotracer

The major emphasis of this research proposal is an understanding of the mechanism of protein synthesis initiation and elongation. Current efforts are focused upon the purification and characterization of each of the components required for these processes. To date seven of these proteins have been purified and characterized (EF-alpha, EF-2, eIF-2, eIF-2A, eIf-4A, eIF-4C, eIF-4D, eIF-5). EF-beta gamma delta , eIF-31, and eIF-4B are being characterized at present. With these proteins, attempts will be made to identify any additional proteins which have been reported to stimulate either model assays or hemoglobin synthesis. In particular, eIF-1, Co-eIF-2A (as described by Gupta and coworkers), ESP (as described by Ochoa and coworkers), and the 24,000 dalton mRNA binding protein (as described by Shatkin and coworkers) will be examined. In addition to the indentification of those protein required for protein synthesis, experiments are in progress to define the biological function of these proteins, either in terms of the sequential utilization of the factors or in terms of defining the interactions a given factor may have with aminoacyl-tRNA, nucleotide triphosphates, mRNA, ribosomes or another factor. Presently, the major area of examination is the recognition and binding of mRNA to 40S subunits and the concomitant requirement for ATP hydrolysis.

本研究建议的主要重点是了解 蛋白质合成起始和延伸的机制。目前的努力 专注于每种成分的纯化和表征 这些过程所需的。 到目前为止，其中七种蛋白质已经被 纯化并表征（EF-α，EF-2，eIF-2，eIF-2A，eIF-4A，eIF-4C， eIF-4D、eIF-5）。 EF-beta gamma delta、eIF-31和eIF-4B正在被 目前的特点。 利用这些蛋白质，将尝试 鉴定任何其他蛋白质，据报道， 模型分析或血红蛋白合成。 特别地，eIF-1、Co-eIF-2A（作为 由Gupta和同事描述），ESP（如Ochoa和同事描述）， 和24，000道尔顿的mRNA结合蛋白（如Shatkin和 同事们将接受检查。 除了鉴定蛋白质所需的蛋白质外， 合成，实验正在进行中，以确定生物功能的 这些蛋白质，无论是在顺序利用的因素， 在定义给定因素可能与 氨酰基-tRNA、核苷酸三磷酸、mRNA、核糖体或另一种因子。 目前，主要研究领域是mRNA的识别和结合 40S亚基和ATP水解的伴随要求。