YEAST PEPTIDE PHEROMONES SYNTHESIS AND MODE OF ACTION
酵母肽信息素的合成和作用方式
基本信息
- 批准号:3270737
- 负责人:
- 金额:$ 16.13万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1978
- 资助国家:美国
- 起止时间:1978-01-01 至 1988-12-31
- 项目状态:已结题
- 来源:
- 关键词:Saccharomyces adenylate cyclase affinity chromatography antibody formation cell adhesion cell cell interaction cell cycle cell fusion cell membrane eukaryote fungal genetics gel electrophoresis gene expression genetic manipulation genetic transcription high performance liquid chromatography membrane activity messenger RNA microorganism conjugation molecular cloning oligopeptides pheromone phosphorylation radioimmunoassay structural genes temperature sensitive mutant
项目摘要
The two haploid cell types (a and Alpha) of the yeast Saccharomyces
cerevisiae each secrete specific peptide hormone-like molecules which
trigger events leading to fusion ("mating") of the cells, a relatively
simple developmental sequence. Because incisive genetic and biochemical
studies can be performed in yeast cells, particular those utilizing
recombinant DNA, it may be possible to learn the precise molecular details
of peptide hormone biosynthesis and mode of action by examining the yeast
mating pheromones (a-factor and Alpha-factor). The approaches to be taken
include: (1) in vitro alterations of the control regions of the cloned
Alpha-factor and a-factor genes, and novel gene fusions to indicator
enzymes, to dissect the cell type-specific regulation of transcription of
these genes; (2) specific antibodies, inhibitors, and mutations, in
conjunction with the cloned genes, to provide probes and substrates for
analyzing the synthesis, membrane translocation, post-translational
processing, and secretory transport of the pheromone precursor proteins
(prepro-Alpha-factor and prepro-a-factor); (3) [125I]-labelled pheromones,
covalent cross-linking of reactive pheromone derivatives, affinity
chromatography using matrix-bound pheromones, putative genes for
Alpha-factor receptor (STE2) and adenylate cyclase (CDC35), and the cloned
STE5 gene, to identify pheromone receptors and to examine the mechanism of
pheromonal modulation of cyclic AMP synthesis; and, (4) transcript mapping,
gene disruption, and DNA sequencing, to characterize the structure and
function of cloned genes whose expression is under pheromonal control.
In multicellular organisms, peptide hormones are responsible for cell-cell
communications that are required during embryonic development and in the
coordination of life functions in adults. The under- or over-production of
particular peptide hormones, or the inability to sense their presence, have
been correlated with numerous human diseases (e.g. dwarfism, Cushing's
disease, and diabetes). Understanding of the fundamental aspects of the
synthesis and release of peptide hormones, and the regulation of these
processes, as well as the biochemical bases of their mechanisms of action,
is necessary for effective diagnosis and therapy of such diseases. In
addition, knowledge of the biosynthesis of yeast pheromones may allow the
production of medically-valuable human hormones by yeast cells on a
commercial scale.
酵母的两种单倍体细胞类型(a和α)
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Jeremy W. Thorner其他文献
Greasing the wheels of secretory transport
润滑分泌转运的车轮
- DOI:
10.1038/35087142 - 发表时间:
2001-08-01 - 期刊:
- 影响因子:19.100
- 作者:
Jeremy W. Thorner - 通讯作者:
Jeremy W. Thorner
Composition and Subunit Structure of Glycerol Kinase from <em>Escherichia coli</em>
- DOI:
10.1016/s0021-9258(18)62117-9 - 发表时间:
1971-06-25 - 期刊:
- 影响因子:
- 作者:
Jeremy W. Thorner;Henry Paulus - 通讯作者:
Henry Paulus
Jeremy W. Thorner的其他文献
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{{ truncateString('Jeremy W. Thorner', 18)}}的其他基金
Mechanisms of gene-specific and genome-wide regulation of mRNA turnover
mRNA 周转的基因特异性和全基因组调控机制
- 批准号:
9042390 - 财政年份:2013
- 资助金额:
$ 16.13万 - 项目类别:
Mechanisms of gene-specific and genome-wide regulation of mRNA turnover
mRNA 周转的基因特异性和全基因组调控机制
- 批准号:
8825521 - 财政年份:2013
- 资助金额:
$ 16.13万 - 项目类别:
ULTRASTRUCTURAL ANALYSIS OF MEMBRANE-ASSOCIATED COMPLEXES IN S CEREVISIAE
酿酒酵母膜相关复合物的超微结构分析
- 批准号:
8362742 - 财政年份:2011
- 资助金额:
$ 16.13万 - 项目类别:
YEAST PEPTIDE PHEROMONES SYNTHESIS AND MODE OF ACTION
酵母肽信息素的合成和作用方式
- 批准号:
3270738 - 财政年份:1978
- 资助金额:
$ 16.13万 - 项目类别:
YEAST PEPTIDE PHEROMONES: SYNTHESIS NAD MODE OF ACTION
酵母肽信息素:合成和作用模式
- 批准号:
3270735 - 财政年份:1978
- 资助金额:
$ 16.13万 - 项目类别:
YEAST PEPTIDE PHEROMONES SYNTHESIS AND MODE OF ACTION
酵母肽信息素的合成和作用方式
- 批准号:
3270736 - 财政年份:1978
- 资助金额:
$ 16.13万 - 项目类别:
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