ULTRASTRUCTURAL ANALYSIS OF MEMBRANE-ASSOCIATED COMPLEXES IN S CEREVISIAE

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 Septins是GTP结合蛋白，由在几乎所有被检测的真核生物的基因组中发现的旁系同源基因编码。这些蛋白质参与质膜内隔室的重塑和划分，例如在细胞分裂期间。 细胞质分裂（Finger，2005）和神经元中树突棘的成熟（Caudron和Barral，2009）。单细胞真核生物酿酒酵母（Saccharomyces cerevisiae）（面包酵母）编码7个septin基因，智人编码14个septin基因（Pan等人，2007年）。任何给定的真核细胞类型通常以细胞类型和发育阶段特异性的方式表达多组septins。在所有已知的情况下，这些基因的蛋白质产物以限定的顺序缔合并组装成线性阵列（McMurray和Thorner，2008 a; Weirich等人，2008年）。此外，所得的线性杂低聚物（“棒”）是具有进行端对端聚合从而形成长丝的能力的结构单元。众所周知，这些细丝与细胞分裂有关，但单靠荧光显微镜还没有揭示足够的细节来彻底理解确切的机制。我们建议使用相关的荧光和X射线成像，以确定在酵母，酿酒酵母中的每个septins的位置。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Septins are GTP-binding proteins encoded by paralogous genes found in the genomes of nearly every eukaryote examined. These proteins are involved in the remodeling of and demarcation of compartments within the plasma membrane, for example during cytokinesis (Finger, 2005) and in the maturation of dendritic spines in neurons (Caudron and Barral, 2009). Unicellular eukaryote Saccharomyces cerevisiae (baker's yeast) encodes seven septin genes, and Homo sapiens fourteen (Pan et al., 2007). Any given eukaryotic cell type expresses multiple sets of septins, often in a cell type- and developmental stage-specific manner. In all cases known, the protein products of those genes associate in a defined order and assemble into a linear array (McMurray and Thorner, 2008a; Weirich et al., 2008). Moreover, the resulting linear hetero-oligomer ("rod") is a building block that has the capacity to undergo end-on-end polymerization, thereby forming filaments. It is known that these filaments are involved with cell division, but fluorescence microscopy alone has not revealed sufficient details to thoroughly understand the exact mechanism. We propose to use correlated fluorescence and x-ray imaging to determine the location of each of the septins in the yeast, Saccharomyces cerevisiae.