ROLE OF INORGANIC POLYPHOSPHATES IN METABOLISM

当前的目标是表征多磷酸酶 (poly（P））新陈代谢，然后将这些信息应用到最终的 目的阐明聚（P）在体内的作用。 这些研究将 由6个部分组成。 (1)多磷酸激酶。 一个程序已经 通过该显影，聚（P）通过电子显微镜清晰可见。 目的是研究聚（P）的结合是否清晰可见， 电镜 目的是研究poly（P） 在电子显微镜下清晰可见。 目的是调查 聚（P）与酶的结合，并使聚（P）可视化， 酶在链的逐步延长过程中。 我们也将 研究poly（P）ATP结合位点的氨基酸序列 激酶。 (2)多磷酸葡糖激酶。 谢氏丙酸杆菌和 最小诺卡氏菌被分类为乙酰菌目， 被认为是原始的生命形式。 聚（P）葡萄糖激酶利用 聚（P）或ATP和N。minima含有另一种酶， 只有ATP。 N.最小值可能代表一个例子，在一个单一的有机体， 作为磷酸化试剂从聚（P）转变为ATP。 葡激酶 嗜热脂肪芽孢杆菌、酵母己糖激酶和大鼠肝 也被调查。 氨基酸序列将使用 5-对氟磺酰基苯甲酰基腺苷作为标记剂。 目的 将是确定是否有相似之处，在ATP网站， 表明从古代到近代进化连续性 进化形式 此外，还将尝试标记和 对聚（P）葡糖激酶的聚（P）位点进行测序。 （三） 内聚磷酸酶。 这种酶或酶将从 酵母，并将确定是否有特异性， 聚（P）的某些大小以及裂解是否产生某些 Poly（P）的长度。 (4)外聚磷酸酶。 已经显示 短链聚（P）中存在一些因子，这是聚合所需的。 利用长链聚（P）。 将确定这一因素。 (5)聚（P）在舍氏疟原虫体内的作用。 使用32 Pi， (P)将通过在乳酸盐培养基中培养细菌来预先标记。 的 将细胞转移到含有未标记的Pi的葡萄糖培养基中。 葡萄糖-6聚（P）和葡萄糖-6聚（P）的比放射性 ATP将被确定以确定聚（P）是否用作 体内磷酸化试剂。 (6)动物细胞中的Poly（P）。 调查将继续进行，以分离并最终证明 poly（P）存在于动物体内。

The immediate objective is to characterize the enzymes of polyphosphates (poly (P)) metabolism and then apply this information to the ultimate objective of elucidating the role of poly (P) in vivo. The studies will consist of 6 parts. (1) Polyphosphate kinase. A procedure has been developed by which poly (P) is clearly visible by electron microscopy. The aim is to investigate the binding of poly (P) is clearly visible by electron microscopy. The aim is to investigate the binding of poly (P) is clearly visible by electron microscopy. The aim is to investigate the binding of poly (P) to the enzyme and visualize the poly (P) and enzyme during the progressive lengthening of the chain. We also will investigate the amino acid sequence of the ATP binding site of poly (P) kinase. (2) Polyphosphate glucokinase. Propionibacterium shermanii and Nocardia minima are classified in the order Acetinomycetales which are considered to be primitive forms of life. Poly (P) glucokinase utilizes either poly (P) or ATP and N. minima contains another enzyme utilizing only ATP. N. minima may represent an example in a single organism of a transition from poly (P) to ATP as a phosphorylating agent. Glucokinase of Bacillus stearothermophilus, yeast hexokinase and of rat liver will also be investigated. The amino acid sequences will be determined using 5-p-fluorosulfonylbenzoyl adenosine as the labeling agent. The purpose will be to determine if there are similarities in the ATP sites which indicate an evolutionary continuity from ancient to more recent evolutionary forms. In addition, an attempt will be made to label and sequence the poly (P) site of poly (P) glucokinase. (3) Endopolyphosphatases. This enzyme or enzymes will be purified from yeast and it will be determined whether or not there is specificity for certain sizes of poly (P) and whether the cleavages yield certain lengths of poly (P). (4) Exopolyphosphatases. It has been shown that some factor is present in short-chain poly (P) which is required for the utilization of long-chain poly (P). This factor will be identified. (5) Role of poly (P) in vivo in P. shermanii. Using 32 P i , the poly (P) will be prelabeled by growing the bacteria in a lactate medium. The cells will be transferred a glucose medium containing unlabeled P i. The specific radioactivity of the glucose-6 poly (P) and the lambda-P of ATP will be determined to ascertain whether poly (P) serves as the phosphorylating agent in vivo. (6) Poly (P) in animal cells. Investigations will be continued to isolate and conclusively demonstrate that poly (P) is present in animals.