ROLE OF INORGANIC POLYPHOSPHATE IN METABOLISM

多年来，人们已经知道无机多聚磷酸盐(Poly P)， 其由长度从3到3的磷酸盐的直链酸酐组成 有200多种磷酸盐残留物存在于微生物中，也存在于 高等动物。聚磷酸盐在新陈代谢中的作用主要是 未知。本研究的目的是探讨Poly P的作用 在丙酸细菌和其他细胞中，如果发展如 以表明这是可取的。丙酸细菌是 特别适合于这样的研究，因为我们已经表明，它们 在某些反应中用无机焦磷酸盐(PPI)代替三磷酸腺苷 而且他们似乎也可能同样利用保利P调查 将分为三大类。1.两个角色的比较 丙酸菌反应中的ATP、PPI和Poly P2.保利 β-葡萄糖激酶：结构、特异性和催化机制。3. 聚P在丙酸菌中的周转。项目1.将包括 三种磷酸盐相对反应速率的测定 化合物，目的是确定Poly P是否具有作用 它还没有被发现，特别是最有可能的来源 项2.关于聚P-葡萄糖激酶将是 初步研究表明，丙酸中含有丰富的这种酶 酸性细菌。该酶的结构和特异性将是 考察了催化作用的机理，即是否 机制是进行性或随机性的。项目3.对公司营业额的计量 Poly P将涉及体内和体外的32P标记和测量 Poly P的标记率。这些结果应该提供一个答案。 关于Poly P是否在新陈代谢中起核心作用的问题。

It has been known for many years that inorganic polyphoshates (Poly P), which consist of linear anhydrides of phoshate varying in length from 3 to more than 200 phosphate residues, are present in microorganisms and also in higher animals. The role of the polyphosphates in metabolism is largely unknown. The object of this research is to investigate the role of Poly P in propionic acid bacteria and in other cells, if developments are such as to indicate this is desirable. The propionic acid bacteria are particularly suitable for such studies since we have shown that they utilize inorganic pyrophosphate (PPi) in certain reactions in place of ATP and it seems likely they likewise may utilize Poly P. The investigation will be divided into 3 broad categories. 1. Comparison of the roles of ATP, PPi and Poly P in reactions of the propionic acid bacteria. 2. Poly P glucokinase: structure, specificity and mechanism of catalysis. 3. Turnover of Poly P in the propionic acid bacteria. Item 1. will consist of measurement of the relative rates of reactions with the three phosphate compounds with the purpose of determining whether or not Poly P has a role which has not yet been discovered and in particular the most likely source of Poly P. Item 2. concerning Poly P-glucokinase will be a continuation of preliminary work which has shown this enzyme is abundant in the propionic acid bacteria. The structure and specificity of the enzyme will be investigated and the mechanism of catalysis, i.e., whether or not the mechanism is processive or random. Item 3., the measurement of turnover of Poly P will involve 32p labeling in vivo and in vitro and measurements of the rate of labeling of the Poly P. These results should provide an answer to the question of whether or not Poly P has a central role in metabolism.