ROLE OF INORGANIC POLYPHOSPHATE IN METABOLISM

无机多磷酸盐在代谢中的作用

• 批准号: 3277240
• 负责人: HARLAND G. WOOD
• 金额: $ 7.35万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-07-01 至 1986-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3277240
• 关键词: Propionibacteriaceae; enzyme structure; gel electrophoresis; glucokinase; glucose metabolism; microorganism metabolism; polyphosphates; propionates; radiotracer; spectrometry

It has been known for many years that inorganic polyphoshates (Poly P), which consist of linear anhydrides of phoshate varying in length from 3 to more than 200 phosphate residues, are present in microorganisms and also in higher animals. The role of the polyphosphates in metabolism is largely unknown. The object of this research is to investigate the role of Poly P in propionic acid bacteria and in other cells, if developments are such as to indicate this is desirable. The propionic acid bacteria are particularly suitable for such studies since we have shown that they utilize inorganic pyrophosphate (PPi) in certain reactions in place of ATP and it seems likely they likewise may utilize Poly P. The investigation will be divided into 3 broad categories. 1. Comparison of the roles of ATP, PPi and Poly P in reactions of the propionic acid bacteria. 2. Poly P glucokinase: structure, specificity and mechanism of catalysis. 3. Turnover of Poly P in the propionic acid bacteria. Item 1. will consist of measurement of the relative rates of reactions with the three phosphate compounds with the purpose of determining whether or not Poly P has a role which has not yet been discovered and in particular the most likely source of Poly P. Item 2. concerning Poly P-glucokinase will be a continuation of preliminary work which has shown this enzyme is abundant in the propionic acid bacteria. The structure and specificity of the enzyme will be investigated and the mechanism of catalysis, i.e., whether or not the mechanism is processive or random. Item 3., the measurement of turnover of Poly P will involve 32p labeling in vivo and in vitro and measurements of the rate of labeling of the Poly P. These results should provide an answer to the question of whether or not Poly P has a central role in metabolism.

多年来已知无机多磷酸盐（Poly P）， 其由长度从3到10变化的线性磷酸酐组成， 超过200种磷酸盐残留物存在于微生物中， 高等动物 多磷酸盐在新陈代谢中的作用主要是 未知 本研究的目的是探讨Poly P 在丙酸细菌和其他细胞中，如果发展如 以表明这是期望的。 丙酸细菌是 特别适合此类研究，因为我们已经证明它们 在某些反应中利用无机焦磷酸盐（PPi）代替ATP 而且他们似乎也可能利用Poly P。 将分为三大类。 1. 的作用比较 ATP、PPi和Poly P在丙酸细菌反应中的作用。 2. 聚 葡糖激酶：结构、特异性和催化机制。 3. 聚P在丙酸细菌中的周转。 项目1.将包括 测量与三种磷酸盐反应的相对速率 化合物的目的是确定聚P是否具有作用 目前还没有被发现，特别是最有可能的来源， Poly P.第2项。关于聚P-葡萄糖激酶将是一个延续， 初步研究表明，这种酶在丙酸 酸性细菌 该酶的结构和特异性将是 研究和催化机理，即，大是大非面前是否 机制是渐进的还是随机的。 项目3.，营业额的计量 Poly P将涉及体内和体外的32 p标记， Poly P的标记率。这些结果应提供答案 Poly P是否在新陈代谢中起核心作用的问题。