ROLE OF INORGANIC POLYPHOSPHATE IN METABOLISM

无机多磷酸盐在代谢中的作用

• 批准号: 3277240
• 负责人: HARLAND G. WOOD
• 金额: $ 7.35万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-07-01 至 1986-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3277240
• 关键词: Propionibacteriaceae; enzyme structure; gel electrophoresis; glucokinase; glucose metabolism; microorganism metabolism; polyphosphates; propionates; radiotracer; spectrometry

It has been known for many years that inorganic polyphoshates (Poly P), which consist of linear anhydrides of phoshate varying in length from 3 to more than 200 phosphate residues, are present in microorganisms and also in higher animals. The role of the polyphosphates in metabolism is largely unknown. The object of this research is to investigate the role of Poly P in propionic acid bacteria and in other cells, if developments are such as to indicate this is desirable. The propionic acid bacteria are particularly suitable for such studies since we have shown that they utilize inorganic pyrophosphate (PPi) in certain reactions in place of ATP and it seems likely they likewise may utilize Poly P. The investigation will be divided into 3 broad categories. 1. Comparison of the roles of ATP, PPi and Poly P in reactions of the propionic acid bacteria. 2. Poly P glucokinase: structure, specificity and mechanism of catalysis. 3. Turnover of Poly P in the propionic acid bacteria. Item 1. will consist of measurement of the relative rates of reactions with the three phosphate compounds with the purpose of determining whether or not Poly P has a role which has not yet been discovered and in particular the most likely source of Poly P. Item 2. concerning Poly P-glucokinase will be a continuation of preliminary work which has shown this enzyme is abundant in the propionic acid bacteria. The structure and specificity of the enzyme will be investigated and the mechanism of catalysis, i.e., whether or not the mechanism is processive or random. Item 3., the measurement of turnover of Poly P will involve 32p labeling in vivo and in vitro and measurements of the rate of labeling of the Poly P. These results should provide an answer to the question of whether or not Poly P has a central role in metabolism.

多年来一直知道无机多形（Poly P）， 由Phoshate的线性赤道组成，长度从3到3 微生物中存在200多个磷酸盐残基，也存在于 更高的动物。 多磷酸盐在代谢中的作用在很大程度上是 未知。 这项研究的目的是研究聚P的作用 在丙酸细菌和其他细胞中，如果有发展 表示这是可取的。 丙酸细菌是 特别适合此类研究，因为我们已经表明他们 利用无机焦磷酸（PPI）代替某些反应代替ATP 而且似乎他们很可能同样可以使用Poly P。调查 将分为三个类别。 1。角色的比较 ATP，PPI和Poly P在丙酸细菌的反应中。 2。poly P葡萄糖激酶：催化的结构，特异性和机制。 3。 丙酸细菌中聚P的营业额。 项目1。将包括 测量与三磷酸反应的相对速率 化合物的目的是确定Poly P是否具有作用 尚未发现，尤其是最有可能的来源 Poly P.项目2。关于多聚P-葡萄糖酶，将延续 表明这种酶在丙泊中很丰富的初步工作 酸性细菌。 酶的结构和特异性将是 研究和催化机制，即是否 机制是过程的或随机的。 项目3.，营业额的测量 Poly P将在体内和体外涉及32p标记和测量 Poly P的标签率。这些结果应提供答案 关于Poly P在代谢中是否具有核心作用的问题。