MUTATIONS AFFECTING THE PRODUCTION OF MATURE RNAS

在我们努力鉴定对前tRNA重要的基因产物时， 在剪接过程中，我们发现了意想不到的蛋白质类别， 可能在调节和亚细胞定位中发挥作用， 剪接酶和前体tRNA底物，以及一种蛋白质 很可能与前转运RNA相互作用。我们的研究RNA 1和 SRN 1链接环境刺激（即，葡萄糖）和RNA加工。 我们 RNA 1和SFP 1的研究将核输入或核结构与RNA 处理. 我们对LOS 1的研究表明， 膜可以影响前tRNA的加工，我们对Stp 1 p的研究提供了 辅助蛋白在催化步骤中的作用的证据 前tRNA剪接。拟议的工作旨在批判性地定义 这些基因产物中的每一种发挥作用的步骤，并确定 它们与大分子相互作用，并由五个具体目标组成。 这些是：（A）确认RNA加工响应于葡萄糖， 研究这种环境信号传输的机制 （B）研究细胞核-细胞质 通讯与RNA加工有关;（C）继续研究RNA 1 以及它在协调环境刺激方面的核心作用， 核-胞质通讯和RNA加工;（D）确定是否 Los 1 p影响核内剪接核酸内切酶的拓扑结构 （E）测试Stp 1 p可以影响前tRNA剪接的模型 核酸内切酶相互作用

In our efforts to identify gene products important to the pre-tRNA splicing process, we discovered unanticipated categories of proteins that are likely to play a role in the regulation and subcellular location of the splicing enzymes and the pre-tRNA substrates, as well as a protein that is likely to interact with the pre-tRNAs. Our studies of RNA1 and SRN1 link environmental stimuli (i.e., glucose) and RNA processing. Our studies of RNA1 and SFP1 link nuclear import or nuclear structure and RNA processing. Our studies of LOS1 indicate that proteins of the nuclear membrane can affect pre-tRNA processing and our studies of Stp1p provide evidence for the role of accessory proteins for the catalytic steps of pre-tRNA splicing. The proposed work is designed to critically define the steps at which each of these gene products functions and to determine the macromolecules with which they interact and consist of five specific aims. These are to: (A) confirm that RNA processing responds to glucose and study the mechanism(s) by which this environmental signal is transmitted to the RNA processing machinery; (B) investigate how nuclear-cytosol communication is involved with RNA processing; (C) continue studying RNA1 and its proposed central role in coordinating environmental stimuli, nuclear-cytosol communication and RNA processing; (D) determine whether Los1p affects the topology of splicing endonuclease in the nuclear membrane; and (E) test the model that Stp1p can affect pre-tRNA-splicing endonuclease interaction.