STRUCTURE OF POLYTENE CHROMOSOME BANDS AND INTERBANDS

最新开发的硬件和软件，以通用的解决方案 重叠时尚，现在允许三维重建 光镜和电子显微镜下的亚细胞结构。 建议将这些三维技术应用于结构 果蝇特异性多线条带和条带间的测定。这 研究将建立在Notch最新的分子比对基础上 5‘调控序列和转录序列到带间和带 多线基因座3C7。 这项研究将从进一步的分子带/带间排列开始 对于具有特定蛋白质叠加的不同遗传基因座 由单抗和亲和纯化的多克隆抗体定位。 在光学和EM上进行详细的三维重建 特定频带/频带间的级别将继续进行。专业软件 将被写来解读和比较复杂的图像。方法 将被开发用于研究三维特定的DNA探针 原位杂交到带/带间。 这个多线染色体的研究将扩展到一个三维的 生物条件下的带/带间重建 修改。转录(泡芙形成)与特定染色体 像差(位置效应、变异和横移)就是例子 用于结构频带/频带间确定。

The recently developed hardware and software, in a generalized resolution overlapping fashion, now allows 3-dimensional reconstruction of subcellular structures at both the light and electron microscopic levels. It is proposed to apply these 3-dimensional techniques to the structural determination of specific Drosophila polytene bands and interbands. This study will build on the recent molecular alignment of the Notch 5'regulatory and transcribed sequences to the interband and band of polytene locus 3C7 respectively. This study will start with further molecular band/interband alignments for diverse genetic loci with a superimposition of specific proteins localized by monoclonal and affinity purified polyclonal antibodies. A detailed, 3-dimensional reconstruction at both the optical and EM levels for specific bands/interbands will proceed. Specialized software will be written to interpret and compare the complicated images. Methods will be developed for the study in 3- dimensions of specific DNa probes in situ hybridized to bands/interbands. This polytene chromosome study will be extended by a 3-dimensional reconstruction of bands/interbands under conditions of biological modification. Transcription (puff formation) and specific chromosome aberrations (position-effect varigation and transvection) are examples for structural band/interband determinations.