STRUCTURE OF POLYTENE CHROMOSOME BANDS AND INTERBANDS

最新开发的硬件和软件，在一个通用的解决方案， 重叠的方式，现在允许三维重建的 在光镜和电镜水平上观察到的亚细胞结构。 建议将这些三维技术应用于结构 确定特定的果蝇多线带和带间。这 这项研究将建立在最近的Notch分子排列的基础上， 5 '调控和转录序列的带间和带 多线位点3C 7。 本研究将从进一步的分子带/带间比对开始 对于不同的遗传基因座， 通过单克隆和亲和纯化的多克隆抗体定位。 一个详细的，三维重建在光学和EM 将继续进行特定频带/频带间的电平。专门的软件 将被写入来解释和比较复杂的图像。方法 将开发用于特定DNa探针的三维研究 与带/带间原位杂交。 这一多线染色体研究将通过一个三维的 生物条件下的带/带间重建 改性转录（粉扑形成）和特定染色体 像差（位置效应变化和横向）就是例子 用于结构带/带间测定。

The recently developed hardware and software, in a generalized resolution overlapping fashion, now allows 3-dimensional reconstruction of subcellular structures at both the light and electron microscopic levels. It is proposed to apply these 3-dimensional techniques to the structural determination of specific Drosophila polytene bands and interbands. This study will build on the recent molecular alignment of the Notch 5'regulatory and transcribed sequences to the interband and band of polytene locus 3C7 respectively. This study will start with further molecular band/interband alignments for diverse genetic loci with a superimposition of specific proteins localized by monoclonal and affinity purified polyclonal antibodies. A detailed, 3-dimensional reconstruction at both the optical and EM levels for specific bands/interbands will proceed. Specialized software will be written to interpret and compare the complicated images. Methods will be developed for the study in 3- dimensions of specific DNa probes in situ hybridized to bands/interbands. This polytene chromosome study will be extended by a 3-dimensional reconstruction of bands/interbands under conditions of biological modification. Transcription (puff formation) and specific chromosome aberrations (position-effect varigation and transvection) are examples for structural band/interband determinations.