STRUCTURE AND FUNCTION OF THE NUCLEAR ENVELOPE

核膜的结构和功能

• 批准号: 3281936
• 负责人: JOHN W SEDAT
• 金额: $ 9.28万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-12-01 至 1986-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3281936
• 关键词: affinity chromatography; chemical structure function; early embryonic stage; electron microscopy; gel electrophoresis; immunological substance; monoclonal antibody; nonmammalian vertebrate embryology; nuclear membrane; oogenesis; surface antigens

This proposal builds on the established library of monoclonal antibodies to Drosophila nuclear protein and the recently developed tools of three-dimensional reconstruction of subcellular structures. This proposal focusses on a subset of the antibodies that label the nuclear envelope. Using electron microscopy, the substructure and location within the nuclear envelope will be established. Further correlation of the nuclear envelope substructures to the interphase chromosomes by the three-dimensional image processing techniques will be accomplished. The antigen will be purified and extensively characterized by biochemical procedures.

该建议建立在已建立的单克隆抗体库的基础上