BIOMEDICAL APPLICATIONS OF LIGHT SCATTER TECHNOLOGY

光散射技术的生物医学应用

• 批准号: 3274292
• 负责人: GARY C SALZMAN
• 金额: $ 22.1万
• 依托单位: UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-09-30 至 1990-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3274292
• 关键词: argon; biomedical equipment development; cell components; computer graphics /printing; computer simulation; computers; electron microscopy; flow cytometry; helium; image processing; interferometry; lasers; leukocytes; light scattering; mathematical model; microorganism classification; model design /development; morphology; neon; optical polarization; particle beam; scanning electron microscopy; single cell analysis; ultraviolet radiation; visible light

Light scattering has become an important probe in flow cytometry because of its sensitivity to both cell size and internal structure. The polarization properties of the scattered light have the potential for providing a wealth of information about cell shape and internal structure. The polarization information is contained in a 4 X 4 Mueller matrix. We propose to exploit a new approach to Mueller matrix measurement made possible by the Zeeman effect laser. This laser nearly artifact-free measurements of the polarization properties of cells. We further propose to exploit a recent development called Fourier transform flow cytometry by which we can use optical modulators in a new way to make precise polarization measurements on cells in suspension and in flow. We propose to apply the recently refined coupled dipole model of the scattering process to bacteria and leukocytes for the purpose of 1) understanding the scattering of light from biological cells and 2) developing clinical laboratory instruments for rapid identification of bacterial and other cells.

光散射已成为流动的重要探测 细胞仪由于其对细胞大小和内部的敏感性而因为 结构。 散射光的极化特性具有 提供有关细胞的大量信息的潜力 形状和内部结构。 极化信息是 包含在4 x 4 Mueller矩阵中。 我们建议利用一种新的方法 Zeeman效应激光使测量成为可能。 这 激光接近极化的无伪影测量 细胞的特性。 我们进一步建议利用最近的 开发称为傅立叶变换流式细胞仪，通过 我们可以以新的方式使用光学调制器来精确 在悬浮液和流动中的细胞上的极化测量。 我们建议应用最近精制的偶联偶极子模型 为此目的的细菌和白细胞的散射过程 1）了解生物细胞的光的散射 2）开发快速的临床实验室工具 鉴定细菌和其他细胞。