GENE REGULATION BY TRANSCRIPTION ANTITERMINATION
通过转录抗终止进行基因调控
基本信息
- 批准号:3276340
- 负责人:
- 金额:$ 32.41万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1981
- 资助国家:美国
- 起止时间:1981-04-01 至 1996-03-31
- 项目状态:已结题
- 来源:
- 关键词:DNA directed RNA polymerase DNA footprinting affinity chromatography bacterial genetics chimeric proteins crosslink gene expression genetic manipulation genetic promoter element genetic recombination host organism interaction immunochemistry laboratory mouse messenger RNA molecular cloning mutant nucleic acid sequence radiation genetics site directed mutagenesis tissue /cell culture transcription termination
项目摘要
The primary goal of this proposal is to elucidate the mechanism of action
of a model antiterminator, the N protein encoded by temperate phage
lambdal. N is a small, modular protein that interacts with a specific
genetic signal (called nut) on the phage genome and a number of host
proteins (called Nus factors) to modify host RNA polymerase to a
termination-resistant mode. The current working model proposes that an
arginine motif present in N binds the boxB RNA hairpin encoded by nut,
bringing N in close contact with the target polymerase. N then captures
polymerase through mRNA looping and collaboration of an adapter host factor
(NusA), and leads RNA polymerase through downstream terminators by being an
operon-specific subunit. Additional host factors interact with conserved
RNA signals and N to facilitate both the assembly as well as persistent
antitermination. The proposed experiments will combine genetic,
biochemical and biophysical approaches to accomplish several major
objectives. The first goal is to carry out several lines of mechanistic
studies to rigorously test various predictions of the model, further
dissecting the role of the core components in termination-suppression and
elucidating the underlying molecular mechanisms. The second goal is to
isolate the missing host factor(s) and their genes by independent genetic
and biochemical approaches and elucidate their function in assembly of the
N-antitermination complex, persistent antitermination, and in switching
phage development from lysogenic to the lytic mode. The third goal is to
study N-boxB interaction with molecular genetic and biophysical approaches
to decipher the chemical principles that govern RNA-protein interaction.
The fourth goal, a new direction of the project, is to identify cellular
genes that are activated by N-like antitermination mechanism, and isolate
potential cellular homologues of N. These studies should provide
fundamental information on the structure, function and regulation of the
prokaryotic transcription apparatus, should illuminate the function of RNA
signals in transcription control, and should reveal aspects of the
molecular basis of sequence-specific recognition of RNA signals by
regulatory proteins.
这项建议的主要目的是阐明作用机制。
由温带噬菌体编码的N蛋白
兰布达尔。N是一种小的、模块化的蛋白质,它与特定的
噬菌体基因组和一些寄主上的遗传信号(称为坚果)
蛋白质(称为NUS因子)将宿主RNA聚合酶修饰为
抗终止模式。目前的工作模式提出,
N中存在的精氨酸基序与坚果编码的boxB RNA发夹结合,
使N与靶聚合酶密切接触。然后,N捕获
通过mRNA环和适配器宿主因子的协作产生的聚合酶
(NusA),并通过作为一种
操纵子特异性亚基。其他宿主因素与保守的
RNA信号和N既便于组装又便于持久
反堕胎。拟议中的实验将结合基因、
生化和生物物理方法来完成几个主要的
目标。第一个目标是进行几条机械化的路线
严格测试模型的各种预测的研究,进一步
剖析了核心组件在终端抑制和
阐明潜在的分子机制。第二个目标是
用独立遗传方法分离缺失宿主因子S及其基因
和生化方法,并阐明它们在组装过程中的作用
N-抗终止复合体、持久抗终止和在切换中
噬菌体从溶源发育到裂解模式。第三个目标是
用分子遗传学和生物物理方法研究N-boxB相互作用
来破译支配RNA-蛋白质相互作用的化学原理。
第四个目标,也是该项目的一个新方向,是识别细胞
被类N-抗终止机制激活的基因,并分离
N的潜在细胞同源物。这些研究应该提供
关于的结构、功能和监管的基本信息
原核转录装置,应阐明RNA的功能
转录控制中的信号,并应该揭示
RNA信号序列特异性识别的分子基础
调节蛋白。
项目成果
期刊论文数量(0)
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{{ truncateString('ASIS DAS', 18)}}的其他基金
GENE REGULATION BY TRASCRIPTION ANTITERMINATION
通过转录抗终止进行基因调控
- 批准号:
2684724 - 财政年份:1981
- 资助金额:
$ 32.41万 - 项目类别:
GENE REGULATION BY TRANSCRIPTION ANTITERMINATION
通过转录抗终止进行基因调控
- 批准号:
3276342 - 财政年份:1981
- 资助金额:
$ 32.41万 - 项目类别:
GENE REGULATION BY TRANSCRIPTION ANTITERMINATION
通过转录抗终止进行基因调控
- 批准号:
3276346 - 财政年份:1981
- 资助金额:
$ 32.41万 - 项目类别:
GENE REGULATION BY TRANSCRIPTION ANTITERMINATION
通过转录抗终止进行基因调控
- 批准号:
3276347 - 财政年份:1981
- 资助金额:
$ 32.41万 - 项目类别:
GENE REGULATION BY TRASCRIPTION ANTITERMINATION
通过转录抗终止进行基因调控
- 批准号:
2175332 - 财政年份:1981
- 资助金额:
$ 32.41万 - 项目类别:
GENE REGULATION BY TRASCRIPTION ANTITERMINATION
通过转录抗终止进行基因调控
- 批准号:
2900537 - 财政年份:1981
- 资助金额:
$ 32.41万 - 项目类别:
GENE REGULATION BY TRANSCRIPTION ANTITERMINATION
通过转录抗终止进行基因调控
- 批准号:
3276343 - 财政年份:1981
- 资助金额:
$ 32.41万 - 项目类别:
GENE REGULATION BY TRANSCRIPTION ANTITERMINATION
通过转录抗终止进行基因调控
- 批准号:
3276344 - 财政年份:1981
- 资助金额:
$ 32.41万 - 项目类别:
GENE REGULATION BY TRASCRIPTION ANTITERMINATION
通过转录抗终止进行基因调控
- 批准号:
2391890 - 财政年份:1981
- 资助金额:
$ 32.41万 - 项目类别:
GENE REGULATION BY TRANSCRIPTION ANTITERMINATION
通过转录抗终止进行基因调控
- 批准号:
3276345 - 财政年份:1981
- 资助金额:
$ 32.41万 - 项目类别:
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