MECHANISM AND REGULATION OF E. COLI RNA POLYMERASE

大肠杆菌RNA聚合酶的机制和调控

• 批准号: 3278120
• 负责人: WILLIAM R MCCLURE
• 金额: $ 30.71万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-07-01 至 1990-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3278120
• 关键词: DNA binding protein; DNA directed RNA polymerase; Escherichia coli; RNA directed DNA polymerase; bacteriophage lambda; chemical binding; enzyme mechanism; fluorescence spectrometry; gene expression; genetic manipulation; genetic regulation; genetic transcription; polymerization; ribosomal RNA; ultracentrifugation

The research proposed will be a systematic biochemical investigation of the mechanism and regulation of E. coli. RNA Polymerase. Several gene regulatory systems will be investigated in vitro. The focus throughout will be to understand how DNA sequences in the control regions of genes contribute to the regulation of transcription initiation. The mechanism of action of several transcriptional activators and repressors will be examined. The effects of DNA supercoiling on selected systems will also be investigated. In two bacteriophage systems, the effects of convergent transcription between two (or more) opposing promoters will be studied. The experiments proposed rely on standard biochemical assays for determining in vitro promoter strength. These results will be complemented in some cases with direct measurements of in vivo RNA chain initiation frequency and in other cases with direct measurements of in vitro binding of RNA polymerase and other regulatory proteins. The answers to the questions we have posed will contribute to the biochemical description of the control of gene expression.

提出的研究将是一个系统的生化调查