MECHANISM AND REGULATION OF E COLI RNA POLYMERASE

这项研究将是一项系统的生化调查， 机制与调控杆菌 RNA聚合酶。 几个基因 将在体外研究调节系统。 重点贯穿始终 就是了解基因控制区的DNA序列 有助于调节转录起始。 的机理 几种转录激活因子和抑制因子的作用将是 考察 DNA超螺旋对所选系统的影响也将在 研究了 在两个噬菌体系统中， 将研究两个（或多个）相对启动子之间的转录。 所提出的实验依赖于标准的生物化学测定， 测定体外启动子强度。 这些成果将得到补充 在某些情况下直接测量体内RNA链起始 频率和在其他情况下直接测量体外结合 RNA聚合酶和其他调节蛋白。 的答案 我们提出的问题将有助于生物化学的描述， 基因表达的控制。

The research proposed will be a systematic biochemical investigation of the mechanism and regulation of E. coli. RNA Polymerase. Several gene regulatory systems will be investigated in vitro. The focus throughout will be to understand how DNA sequences in the control regions of genes contribute to the regulation of transcription initiation. The mechanism of action of several transcriptional activators and repressors will be examined. The effects of DNA supercoiling on selected systems will also be investigated. In two bacteriophage systems, the effects of convergent transcription between two (or more) opposing promoters will be studied. The experiments proposed rely on standard biochemical assays for determining in vitro promoter strength. These results will be complemented in some cases with direct measurements of in vivo RNA chain initiation frequency and in other cases with direct measurements of in vitro binding of RNA polymerase and other regulatory proteins. The answers to the questions we have posed will contribute to the biochemical description of the control of gene expression.