CONTROL OF VIRUS INDUCED LYSIS

探索、检测和修饰细胞蛋白质特征的能力 表面是许多诊断和 治疗策略 一个主要障碍是， 蛋白质-膜相互作用的性质在很大程度上是未知的。 两个模型 已经开发了使用分子遗传学来确定 调节这种相互作用的原则： 噬菌体λ和噬菌体PhiX 174的E。 这些基因很小 （S = 107个密码子，E = 91个密码子）和致死性，因此非常适合于 突变分析 体外mRNA和蛋白质合成将被起诉， 分别和成对地描述来自一个 收集了约50个膜相互作用方面的错义S突变体 和蛋白酶可及性。 许多这些S 突变体将被分离和测序，以确定相互作用域 在多肽中。 新型的平移控制系统S将 通过定点诱变进行探测，以确定参数， 这两种不同的蛋白质产物的合成受到调节， 比例 E基因将进行精细结构lac融合 分析和回归研究，以确定以下方面的最低要求： 多肽的膜插入。 E基因的错义分析 将以同样的方式进行成功地为S顺反子。

The ability to explore, detect and modify the protein features of cell surfaces is a key in the long-term development of many diagnostic and therapeutic strategies. A major obstacle is the fact that the fundamental nature of protein-membrane interactions is largely unknown. Two model systems have been developed for using molecular genetics to determine the principles regulating such interactions: the lysis control genes S of bacteriophage lambda and E of bacteriophage PhiX174. These genes are small (S = 107 codons, E = 91 codons) and lethal, and thus are well-suited for mutational analysis. In vitro mRNA and protein synthesis will be sued to characterize both individually and in pairs representatives from a collection of about 50 missense S mutants in terms of membrane interaction and protease accessibility. Intragenic suppressors of many of these S mutants will be isolated and sequenced to determine interaction domains within the polypeptide. The novel translational control system of S will be probed by site-directed mutagenesis, to determine the parameters by which the two different protein products are synthesized in regulated proportion. The E gene will be subjected to fine structure lac fusion analysis and reversion studies to determine the minimum requirements for membrane insertion of the polypeptide. A missense analysis of the E gene will be carried out in the same way done successfully for the S cistron.