Replacement fluorescence activated cell sorter for the Jenner Laboratory (BBSRC National Vaccinology Centre: The Jenner Building)

詹纳实验室的替换荧光激活细胞分选机（BBSRC 国家疫苗学中心：詹纳大楼）

• 批准号: BB/V019031/1
• 负责人: Simon Graham
• 金额: $ 55.36万
• 依托单位: The Pirbright Institute
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FV019031%2F1
• 关键词: Replacement; fluorescence; activated; cell; sorter

Fluorescence-activated cell sorting (FACS) is a powerful technology that enables the measurement of characteristics of single cells and then separates these cells based on their characteristics. Tens of thousands of individual cells can be isolated within minutes and are then available for analysis. This is critical to our understanding of how animals respond to virus infections and enable us to design and develop viral vaccines to prevent disease.The Pirbright Institute's (Pirbright) purpose is to predict, detect, understand, and respond to serious viral diseases of livestock and viruses that spread from animals to humans. Pirbright is unique within the UK, providing scientists with access to both state-of-the-art animal and laboratory facilities to research infectious diseases of livestock at high containment levels. The BBSRC National Vaccinology Centre (Jenner Building) at Pirbright, is a low containment facility that supports work with medium risk viruses. This project aims to ensure continuity of the FACS capability in the Jenner Building for the next 10-12 years. To achieve this, we propose to replace our current 15 years old FACSorter, the BD FACSAria U3 (Aria U3), positioned within the Jenner Building, with an upgrade to a BD FACSAria Fusion (Aria Fusion).The Aria Fusion is superior to the Aria U3 with exceptional multicolour performance, up to date software and hardware and enhanced biosafety protection. The addition of the microbiological safety cabinet will increase sample and operator protection. Retaining our FACS capacity and expanding our ability to sort virus infected samples will enable us to understand better animal responses to virus infection.

流式细胞仪（FACS）是一种强大的技术，能够测量单细胞的特性，然后根据它们的特性分离这些细胞。数万个单个细胞可以在几分钟内分离出来，然后用于分析。这对我们了解动物如何应对病毒感染至关重要，并使我们能够设计和开发病毒疫苗来预防疾病。皮尔布莱特研究所（Pirbright Institute）的目的是预测、检测、了解和应对严重的牲畜病毒性疾病和从动物传播到人类的病毒。Pirbright在英国是独一无二的，为科学家提供了最先进的动物和实验室设施，以研究高遏制水平的牲畜传染病。位于Pirbright的BBSRC国家疫苗学中心（詹纳大楼）是一个低防护设施，支持中等风险病毒的工作。该项目旨在确保詹纳大楼外地资产管制系统能力在今后10至12年内的连续性。为了实现这一目标，我们建议更换我们目前使用了15年的FACSorter，即位于詹纳大楼内的BD FACSAria U3（Aria U3），升级为BD FACSAria Fusion（Aria Fusion）。Aria Fusion上级于Aria U3，具有出色的多色性能、最新的软件和硬件以及增强的生物安全保护。增加微生物安全柜将增加对样品和操作员的保护。保留我们的流式细胞仪的能力和扩大我们的能力，排序病毒感染的样品将使我们能够更好地了解动物对病毒感染的反应。

项目成果

Distinct effector functions mediated by Fc regions of bovine IgG subclasses and their interaction with Fc gamma receptors.

• DOI: 10.3389/fimmu.2023.1286903
• 发表时间: 2023
• 期刊: Frontiers in immunology
• 影响因子: 7.3

Preventing bacterial disease in poultry in the post-antibiotic era: a case for innate immunity modulation as an alternative to antibiotic use.

• DOI: 10.3389/fimmu.2023.1205869
• 发表时间: 2023
• 期刊: Frontiers in immunology
• 影响因子: 7.3

Inhibition of Marek's Disease Virus Replication and Spread by 25-hydroxycholesterol and 27-hydroxycholesterol In Vitro.

• DOI: 10.3390/v15081652
• 发表时间: 2023-07-29
• 期刊: Viruses
• 作者: Kamble N;Reddy VRAP;Jackson B;Anjum FR;Ubachukwu CC;Patil A;Behboudi S
• 通讯作者: Behboudi S

Marek's disease virus-specific T cells proliferate, express antiviral cytokines but have impaired degranulation response.

• DOI: 10.3389/fimmu.2022.973762
• 发表时间: 2022
• 期刊: Frontiers in immunology
• 影响因子: 7.3

Marek's Disease Virus Modulates T Cell Proliferation via Activation of Cyclooxygenase 2-Dependent Prostaglandin E2.

• DOI: 10.3389/fimmu.2021.801781
• 发表时间: 2021
• 期刊: Frontiers in immunology
• 影响因子: 7.3
• 作者: Kamble N;Gurung A;Kaufer BB;Pathan AA;Behboudi S
• 通讯作者: Behboudi S