STRUCTURE AND FUNCTION OF E. COLI POL A GENE
STRUCTURE AND FUNCTION OF E. COLI POL A GENE
批准号:
3275805
负责人:
NIGEL David GRINDLEY
金额:
$16.06万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1980
资助国家:
美国
项目状态:
已结题
起止时间:
1980-05-01 至 1986-04-30
关键词:
DNA directed RNA polymerase DNA repair Escherichia coli alleles bacterial DNA bacterial genetics bacteriophage lambda computer data analysis gene expression gene mutation genetic manipulation genetic mapping genetic transcription molecular cloning mutant nucleic acid sequence radiotracer structural genes
中文摘要
DNA聚合酶I (Pol I)是大肠杆菌中起重要作用的酶
英文摘要
DNA polymerase I (Pol I) of E. coli is an enzyme that plays an important
role in the cell both during replication, in the processing of Okazaki
fragments, and in the repair of damaged DNA. It is arguably the best model
system for the study of the molecular details of polymerase action, and for
an investigation of the way in which processive enzymes can co-ordinate
synthesis with movement along a macromolecule. Our recent work has
provided the tools for a detailed analysis of the mechanism of action of
Pol I: we have determined the sequence of the structural gene (po1A),
constructed strains which overproduce a functional polymerase fragment
suitable for structural studies, and established a system for the rapid
mapping, cloning and sequencing of po1A mutants. Most of the work
described in this proposal is designed to complement the X-ray
crystallographic studies of our collaborators, by locating the DNA binding
site and assigning functions to regions of the Pol I molecule. We shall
define the interaction between Pol I and its DNA substrate both nby
affinity labeling the protein, and by chemical and enzymatic probing of
contacts on the DNA molecule. We shall identify functionally important
regions of Pol I by sequencing and enzymatic studies of po1A mutants. In
addition, we intend to examine aspects of the biological role of the po1A
gene, determining whether the gene is essential, what factors control the
intracellular level of Pol I, and whether Pol I functions in association
with other proteins. While this project has no direct health-related
application, it is clear that an improved understanding of those processes
in DNA replication and repair that maintain the integrity of the genome
should eventually provide insights into the mechanisms of mutagenesis and
thus of carcinogenesis.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
EXPRESSION AND SCALED-UP PREPARATION OF THE HIV INTEGRASE
-
批准号:6107539
-
项目类别:
-
资助金额:$3.99万
-
财政年份:1997
-
负责人:NIGEL David GRINDLEY
-
依托单位:
EXPRESSION AND SCALED-UP PREPARATION OF THE HIV INTEGRASE
-
批准号:6296698
-
项目类别:
-
资助金额:$3.99万
-
财政年份:1996
-
负责人:NIGEL David GRINDLEY
-
依托单位:
GORDON CONFERENCE ON BIOL. REGULATORY MECHANISMS
-
批准号:3434902
-
项目类别:
-
资助金额:$0.5万
-
财政年份:1985
-
负责人:NIGEL David GRINDLEY
-
依托单位:
MECHANISM OF INSERTION SEQUENCE TRANSLOCATION
-
批准号:3275751
-
项目类别:
-
资助金额:$21.07万
-
财政年份:1980
-
负责人:NIGEL David GRINDLEY
-
依托单位:
MECHANISM OF INSERTION SEQUENCE TRANSLOCATION
-
批准号:3275752
-
项目类别:
-
资助金额:$20.5万
-
财政年份:1980
-
负责人:NIGEL David GRINDLEY
-
依托单位:
Structure and Function of DNA Polymerase I of E.coli
-
批准号:6890870
-
项目类别:
-
资助金额:$54.36万
-
财政年份:1980
-
负责人:NIGEL David GRINDLEY
-
依托单位:
Structure and Function of DNA Polymerase I of E.coli
-
批准号:6742538
-
项目类别:
-
资助金额:$52.84万
-
财政年份:1980
-
负责人:NIGEL David GRINDLEY
-
依托单位:
Structure and function of DNA Polymerase I of E.coli
-
批准号:7260180
-
项目类别:
-
资助金额:$57.23万
-
财政年份:1980
-
负责人:NIGEL David GRINDLEY
-
依托单位:
Structure and function of DNA Polymerase I of E.coli
-
批准号:7616682
-
项目类别:
-
资助金额:$58.95万
-
财政年份:1980
-
负责人:NIGEL David GRINDLEY
-
依托单位:
MECHANISM OF INSERTION SEQUENCE TRANSLOCATION
-
批准号:2175191
-
项目类别:
-
资助金额:$38.39万
-
财政年份:1980
-
负责人:NIGEL David GRINDLEY
-
依托单位:
STRUCTURE AND FUNCTION OF E COLI POLA GENE
-
批准号:2175207
-
项目类别:
-
资助金额:$33.16万
-
财政年份:1980
-
负责人:NIGEL David GRINDLEY
-
依托单位:
STRUCTURE/FUNCTION OF DNA POLYMERASE I OF E COLI
-
批准号:2175209
-
项目类别:
-
资助金额:$36.71万
-
财政年份:1980
-
负责人:NIGEL David GRINDLEY
-
依托单位:
MECHANISM OF INSERTION SEQUENCE TRANSLOCATION
-
批准号:6018524
-
项目类别:
-
资助金额:$43.59万
-
财政年份:1980
-
负责人:NIGEL David GRINDLEY
-
依托单位:
Structure and Function of DNA Polymerase I of E.coli
-
批准号:7057758
-
项目类别:
-
资助金额:$54.58万
-
财政年份:1980
-
负责人:NIGEL David GRINDLEY
-
依托单位:
STRUCTURE/FUNCTION OF DNA POLYMERASE I OF E COLI
-
批准号:6519025
-
项目类别:
-
资助金额:$44.71万
-
财政年份:1980
-
负责人:NIGEL David GRINDLEY
-
依托单位:
Mechanism of Insertion Sequence Translocation
-
批准号:6887409
-
项目类别:
-
资助金额:$40.88万
-
财政年份:1980
-
负责人:NIGEL David GRINDLEY
-
依托单位:
Structure and function of DNA Polymerase I of E.coli
-
批准号:7821218
-
项目类别:
-
资助金额:$60.01万
-
财政年份:1980
-
负责人:NIGEL David GRINDLEY
-
依托单位:
Structure and function of DNA Polymerase I of E.coli
-
批准号:7408624
-
项目类别:
-
资助金额:$57.29万
-
财政年份:1980
-
负责人:NIGEL David GRINDLEY
-
依托单位:
STRUCTURE AND FUNCTION OF E COLI POLA GENE
-
批准号:3275811
-
项目类别:
-
资助金额:$27.27万
-
财政年份:1980
-
负责人:NIGEL David GRINDLEY
-
依托单位:
STRUCTURE AND FUNCTION OF E COLI POLA GENE
-
批准号:3275804
-
项目类别:
-
资助金额:$26.13万
-
财政年份:1980
-
负责人:NIGEL David GRINDLEY
-
依托单位:
海外基金