SUBUNIT/DOMAIN INTERACTIONS IN MULTIFUNCTIONAL ENZYMES

化学反应序列的催化作用及其整合与调控 细胞的代谢途径取决于几个阶段的组织。 不同的层次。其中包括蛋白质组分的组织 在细胞水平上的各种细胞器和隔室，联结 将特定的蛋白质亚基转化为酶或多酶复合体 特定蛋白质结构域的组织和关联 多肽亚基的结构。这个项目的长期目标是 是定义亚单位和结构域相互作用在结构中的作用 以及多功能酶复合体的功能。通过利用特定的 色氨酸途径或脉孢子虫的多功能酶复合体 可以进行化学、物理和遗传分析的Crassa，我们 建议探索这一多功能中的域结构和组织 蛋白质及其结构域和亚单位相互作用在细胞周期中的作用 酶活性的表达。

The catalysis of reaction sequences and their integration and regulation in the metabolic pathways of the cell depends on organization at several different levels. These include the organization of protein components in the various organelles and compartments at the cell level, the association of specific protein subunits into enzymes or multienzyme complexes and the organization and association of specific protein domains within the structure of the polypeptide subunits. The long range goal of this project is to define the role of subunit and domain interactions in the structure and function of multifunctional enzyme complexes. By utilizing a specific multi-functional enzyme complex from the tryptophan pathway or Neurospora crassa which is amenable to chemical, physical and genetic analysis, we propose to probe domain structure and organization in this multifunctional protein and the role of the interaction of domains and subunits in the expression of enzyme activities.