SUBUNIT/DOMAIN INTERACTIONS IN MULTIFUNCTIONAL ENZYMES

反应序列的催化作用及其整合与调控 细胞的代谢途径取决于几个方面的组织， 不同层次 这些包括蛋白质成分的组织， 细胞水平上的各种细胞器和隔室， 将特定蛋白质亚基转化为酶或多酶复合物， 组织和协会内的特定蛋白质结构域 多肽亚基的结构。 本项目的长期目标 是定义亚基和结构域相互作用在结构中的作用 多功能酶复合物的功能。 通过使用特定的 来自色氨酸途径或脉孢菌属的多功能酶复合物 crassa是经得起化学，物理和遗传分析，我们 建议在这个多功能的领域中探索领域结构和组织 蛋白质的结构域和亚基的相互作用， 酶活性的表达。

The catalysis of reaction sequences and their integration and regulation in the metabolic pathways of the cell depends on organization at several different levels. These include the organization of protein components in the various organelles and compartments at the cell level, the association of specific protein subunits into enzymes or multienzyme complexes and the organization and association of specific protein domains within the structure of the polypeptide subunits. The long range goal of this project is to define the role of subunit and domain interactions in the structure and function of multifunctional enzyme complexes. By utilizing a specific multi-functional enzyme complex from the tryptophan pathway or Neurospora crassa which is amenable to chemical, physical and genetic analysis, we propose to probe domain structure and organization in this multifunctional protein and the role of the interaction of domains and subunits in the expression of enzyme activities.