SUBUNIT/DOMAIN INTERACTIONS IN MULTIFUNCTIONAL ENZYMES

多功能酶中的亚基/结构域相互作用

• 批准号: 3275108
• 负责人: JOHN A DE MOSS
• 金额: $ 1.77万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-05-01 至 1986-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3275108
• 关键词: Neurospora; chemical structure function; enzyme complex; enzyme structure; enzyme substrate; gel electrophoresis; genes; genetic manipulation; glutamine; high performance liquid chromatography; molecular cloning; mutant; o aminobenzoate; oxoacid lyase; tryptophan

The catalysis of reaction sequences and their integration and regulation in the metabolic pathways of the cell depends on organization at several different levels. These include the organization of protein components in the various organelles and compartments at the cell level, the association of specific protein subunits into enzymes or multienzyme complexes and the organization and association of specific protein domains within the structure of the polypeptide subunits. The long range goal of this project is to define the role of subunit and domain interactions in the structure and function of multifunctional enzyme complexes. By utilizing a specific multi-functional enzyme complex from the tryptophan pathway or Neurospora crassa which is amenable to chemical, physical and genetic analysis, we propose to probe domain structure and organization in this multifunctional protein and the role of the interaction of domains and subunits in the expression of enzyme activities.

反应序列的催化作用及其整合与调控 细胞的代谢途径取决于几个方面的组织， 不同层次 这些包括蛋白质成分的组织， 细胞水平上的各种细胞器和隔室， 将特定蛋白质亚基转化为酶或多酶复合物， 组织和协会内的特定蛋白质结构域 多肽亚基的结构。 本项目的长期目标 是定义亚基和结构域相互作用在结构中的作用 多功能酶复合物的功能。 通过使用特定的 来自色氨酸途径或脉孢菌属的多功能酶复合物 crassa是经得起化学，物理和遗传分析，我们 建议在这个多功能的领域中探索领域结构和组织 蛋白质的结构域和亚基的相互作用， 酶活性的表达。

项目成果

Deletion analysis of domain independence in the TRP1 gene product of Neurospora crassa.

粗糙脉孢菌 TRP1 基因产物中结构域独立性的缺失分析。

• DOI: 10.1007/bf00315796
• 发表时间: 1990
• 期刊: Molecular & general genetics : MGG
• 作者: Walker,MS;DeMoss,JA
• 通讯作者: DeMoss,JA

Neurospora tryptophan synthase. Characterization of the pyridoxal phosphate binding site.

脉孢菌色氨酸合酶。

• 发表时间: 1988
• 期刊: The Journal of biological chemistry
• 作者: Pratt,ML;DeMoss,JA
• 通讯作者: DeMoss,JA

Anthranilate synthase of Neurospora crassa: reaction and labeling with glutamine analogs.

粗糙脉孢菌的邻氨基苯甲酸合酶：用谷氨酰胺类似物进行反应和标记。

• DOI: 10.1016/0003-9861(82)90366-6
• 发表时间: 1982
• 期刊: Archives of biochemistry and biophysics
• 影响因子: 3.9
• 作者: Paukert,JL;Henkin,J;KeeseyJr,J;DeMoss,JA
• 通讯作者: DeMoss,JA

Orientation of enzymic domains in tryptophan synthase of Neurospora crassa: an immunoblot analysis of TRP3 mutant products.

粗糙脉孢菌色氨酸合酶中酶结构域的方向：TRP3 突变产物的免疫印迹分析。

• DOI: 10.1007/bf00328130
• 发表时间: 1987
• 期刊: Molecular & general genetics : MGG
• 作者: Matchett,WH;Lacy,AM;DeMoss,JA
• 通讯作者: DeMoss,JA

Organization of the functional domains of anthranilate synthase from Neurospora crassa. Limited proteolysis studies.

粗糙脉孢菌邻氨基苯甲酸合酶功能域的组织。

• 发表时间: 1986
• 期刊: The Journal of biological chemistry
• 作者: Walker,MS;DeMoss,JA
• 通讯作者: DeMoss,JA