STUDIES OF DNA POLYMERASES

培养人DNA聚合酶α、β和γ的种类 成纤维细胞和HeLa细胞将被分级分离并研究 保真度、催化性能和功能。 将特别强调 在成纤维细胞的晚期传代数量期间发生的变化，或 在生长至汇合期间。 抗体研究将在 为了尝试设计亲和柱， 净化和理解的意义和共性 各种阿尔法亚种 人类聚合酶将被测试， 各种底物，以确定它们在DNA修复中的作用， 确定可能与聚合酶一起发挥这种作用的因素。 E. 大肠杆菌DNA聚合酶也将进行研究。 一种新的聚合酶I 将表征recA诱导后出现的活动， 聚合酶II和III在各种受损底物上的表达将被破坏。 确定。

Species of DNA polymerases Alpha, Beta, and Gamma from cultured human fibroblasts and HeLa cells will be fractionated and studied with regard to fidelity, catalytic properties, and function. Particular emphasis will be put upon changes that occur during late passage numbers of fibroblasts or during growth to confluence. Antibody studies will be carried out for the Alpha-species, in order to attempt to design affintiy columns for purification and to understand the significance and commonalities of the various Alpha-subspecies. The human polymerases will be tested upon various substrates in order to ascertain their role in DNA repair and to identify factors that may work with the polymerases in this capacity. E. coli DNA polymerases will also be studied. A new species of polymerase I that appears after recA induction will be characterized and the activities of polymerases II and III upon various damaged substrates will be ascertained.