CONFORMATIONS OF GLYCOPEPTIDES AND GLYCOPROTEINS

本研究的目的是揭示中国古代文学的一般特征， 糖肽及其相关寡糖的构象。 我们将 探索生物功能与不同的 糖肽键和寡糖共价结构的类型。 这一目标将通过应用核磁共振技术来实现 光谱和构象能计算到一些特定的， 充分表征的模型糖肽、糖蛋白和寡糖 系统的选择是因为它们的简单性和可用性， 足够的纯度和数量用于NMR实验。 模型系统将 包括南极鱼血液中的抗冻糖蛋白，氨基 人乳中的含糖低聚糖，甲壳质低聚糖 和胎球蛋白的糖肽。 核磁共振实验将包括酰胺 通过溶剂饱和转移测定的质子交换率，线 通过顺磁自旋标记和核Overhauser效应加宽。 糖肽和寡糖的构象计算将 利用蒙特卡罗方法搜索二面角， 构象空间，其中势能由 经验能量函数

The purpose of this research is to discover the general features of the conformation of glycopeptides and their related oligosaccharides. We will explore the relationship between biological function and the different types of glycopeptide linkage and of oligosaccharide covalent structure. This goal will be achieved by applying nuclear magnetic resonance spectroscopy and conformational energy calculations to a few specific and well characterized model glycopeptides, glycoproteins and oligosaccharide systems which are chosen for their simplicity and availabiltiy in sufficient purity and quantity for nmr experiments. The model systems will include antifreeze glycoprotein from the blood of Antarctic fish, amino sugar containing oligosaccharides from human milk, chitin oligosaccharides and the glycopeptide of fetuin. The nmr experiments will include amide proton exchange rates measured by transfer of solvent saturation, line broadening by paramagnetic spin label and nuclear Overhauser effect. Conformational calculations in glycopeptides and oligosaccharides will utilize Monte Carlo methods to search the dihedral angles in a conformational space in which potential energies are constructed from empirical energy functions.