"Painting" the 3D proteome: folding, conformation and interactions

“绘制”3D 蛋白质组：折叠、构象和相互作用

• 批准号: DP230101050
• 负责人: Prof Daniel Hatters
• 金额: $ 38.31万
• 依托单位: The University of Melbourne
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2023
• 资助国家: 澳大利亚
• 起止时间: 2023-01-01 至 2025-12-31
• 项目状态: 未结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP230101050
• 关键词: Painting; 3D; proteome; folding; conformation

The project aims to develop a "residue painting approach", employing novel chemical biology reagents and advanced quantitative proteomics, to monitor changes in protein folding, conformations and interactions in cells, in response to stimuli. Proteins direct almost all functions required to sustain life. The project expects to map the dynamic 3D-structures of thousands of proteins that inform the networks they are in, and of the conformations they adopt. Expected outcomes include the development of novel biotechnology tools for protein structure and function analysis, the illumination of important cell biology pathways underpinning molecular responses to stimuli and stress, and the training of our next generation of scientists.

该项目旨在开发一种“残留物绘画方法”，采用新型化学生物学试剂和先进的定量蛋白质组学，来监测细胞中蛋白质折叠、构象和相互作用对刺激的反应变化。蛋白质控制着维持生命所需的几乎所有功能。该项目预计将绘制数千种蛋白质的动态3D结构，这些蛋白质为它们所处的网络以及它们所采用的构象提供信息。预期成果包括开发用于蛋白质结构和功能分析的新型生物技术工具，阐明支持对刺激和压力的分子反应的重要细胞生物学途径，以及培养我们的下一代科学家。