Exploring the chopping board microbiome

探索砧板微生物组

• 批准号: BB/W008955/1
• 负责人: Alan Goddard
• 金额: $ 4万
• 依托单位: Aston University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FW008955%2F1
• 关键词: Exploring; chopping; board; microbiome

Many foodborne infections begin in the home, often through poor hygiene where chopping boards provide an opportunity for raw foods to cross-contaminate. By undertaking microbiological analysis of chopping boards, including investigating the antimicrobial resistance profiles of the organisms found, we will address two of the areas of the priority area "assuring food safety and standards":How can the FSA better understand and reduce the impact of foodborne pathogens?How can the FSA improve the evidence base concerning antimicrobial resistance (AMR) and food?We will use a combination of contributary, collaborative and co-creation citizen science approaches whereby we will use our undergraduate Bioscience students as ambassadors to hard-to-reach communities. Ambassadors and citizens will be involved in the design of protocols and materials, as well as the final dissemination outputs including educational materials for communities and academic papers. We have six work packages that allow us to achieve our objectives.WP1 - Recruitment of citizen scientists. Our Biosciences students undertake microbiological practical work as a core part of their programme. Through our student community, we will recruit ~150-200 ambassadors who will give us privileged access to their homes and communities, as well as providing rapid sample collection as they will be travelling to campus regularly. Samples will be collected by our participants using the ambassadors as facilitators.WP2 - Co-creation of sampling methods. With a subset of our ambassadors and their households, we will co-create our final sampling methods. These will be designed to be robust and reproducible whilst providing sound scientific outcomes and being acceptable to the end users. We will co-create the instructional material to ensure it is effective and fit-for-purpose.WP3 - Chopping board sampling. Ambassadors will collect sampling kits and engage their households with sampling. Alongside this, they will record the relevant demographic information as well as key parameters relating to chopping board use and behavioural information.WP4 - Sample analysis. This will be conducted either by our project manager or by the participants themselves at "open sessions" where they can visit the laboratory and undertake the microbiological testing themselves.WP6 - Dissemination. We will use social media, including "takeovers" by our citizens to publicise the project throughout. The academic team and our ambassadors will run an end-of-project showcase for our citizens and the wider community to present the project and its outcomes. Ultimately, we want to develop best practice guidance for chopping board use for each community. Using our scientific findings, our ambassadors and citizens will co-create, with the academic team, educational materials specifically targeted to our community groups for distribution to promote behavioural change. We will publish the microbiological and citizen science outcomes of the project with our ambassadors and citizens involved in writing the manuscripts and named as authors as appropriate.WP7 - Monitoring and evaluation. We will quantify project success in terms of number of engagements (ambassadors and participants recorded separately). Our project manager will replicate key results to ensure scientific reproducibility of our findings. We will evaluate the societal benefits through electronic surveys and individual interviews. For our participants we will assess changes in food hygiene knowledge and behaviour and, for our ambassadors, development in their knowledge of citizen science.

许多食源性感染开始在家里，往往通过卫生条件差，砧板提供了一个机会，生的食物交叉污染。通过对砧板进行微生物分析，包括调查发现的微生物的耐药性，我们将解决优先领域“确保食品安全和标准”的两个领域：食品标准局如何更好地了解和减少食源性病原体的影响？FSA如何改善关于抗菌素耐药性（AMR）和食品的证据基础？我们将使用贡献，协作和共同创造公民科学方法的组合，我们将使用我们的本科生物科学学生作为大使难以到达的社区。大使和公民将参与协议和材料的设计，以及最后的传播产出，包括社区教育材料和学术论文。我们有六个工作包，使我们能够实现我们的目标。WP 1-公民科学家的招聘。我们的生物科学学生承担微生物实践工作作为其计划的核心部分。通过我们的学生社区，我们将招募约150-200名大使，他们将让我们有特权进入他们的家庭和社区，并提供快速样本收集，因为他们将定期前往校园。我们的参与者将利用大使作为促进者收集样本。WP 2-共同创建采样方法。与我们的大使和他们的家庭的子集，我们将共同创建我们的最终抽样方法。这些将被设计为稳健和可重现的，同时提供合理的科学结果，并为最终用户所接受。我们将共同创建的教学材料，以确保它是有效的，适合的目的。WP 3-砧板抽样。大使们将收集采样工具包，并让他们的家庭参与采样。除此之外，他们还将记录相关的人口统计信息以及与砧板使用和行为信息有关的关键参数。这将由我们的项目经理或参与者自己在“公开会议”上进行，他们可以参观实验室并自己进行微生物检测。WP 6-传播。我们将利用社交媒体，包括公民的“接管”，在整个过程中宣传该项目。学术团队和我们的大使将为我们的公民和更广泛的社区举办一个项目结束展示，以展示该项目及其成果。最终，我们希望为每个社区的砧板使用制定最佳实践指南。利用我们的科学研究成果，我们的大使和公民将与学术团队共同创作专门针对我们社区团体的教育材料，以促进行为改变。我们将公布该项目的微生物和公民科学成果，我们的大使和公民参与撰写手稿，并酌情命名为作者。我们将量化项目成功的参与次数（大使和参与者分别记录）。我们的项目经理将复制关键结果，以确保我们发现的科学可重复性。我们将通过电子调查和个人访谈评估社会效益。对于我们的参与者，我们将评估食品卫生知识和行为的变化，对于我们的大使，他们的公民科学知识的发展。