SITE SPECIFIC MUTATION IN METALLOTHIONEIN

金属硫蛋白的位点特异性突变

• 批准号: 3281615
• 负责人: PC C. HUANG
• 金额: $ 18.6万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-01-01 至 1991-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3281615
• 关键词: chemical structure function; cyclic aminoacid; gene expression; genetic transcription; genetic translation; metallothionein; nucleic acid sequence; protein engineering; protein sequence

Site specific mutations in the metallothionein coding sequence will be followed for their expression. Selected codons will be specifically altered to give rise to amino acid replacement from non-aromatic to aromatic. The latter is normally absent in all native metallothioneins thus far analyzed. The possibility that the absence of aromatic amino acids is due to mutational probability of unfavorable processing at the transcriptional or translational level will be examined in vivo and in vitro. The altered metallothionein if obtained will be compared with the function and properties of those occurring naturally.

在金属硫蛋白编码序列中的位点特异性突变将是 跟随他们的表情。 选择的密码子将具体地 改变以引起氨基酸从非芳香族替换为 芳香的 后者通常在所有天然金属硫蛋白中不存在 到目前为止，分析。 芳香族氨基酸的缺失可能是由于 在转录水平上不利加工的突变概率， 将在体内和体外检查翻译水平。 改变的 如果获得金属硫蛋白，将与功能进行比较， 这些自然发生的事情。