SITE SPECIFIC MUTATION IN METALLOTHIONEIN

金属硫蛋白的位点特异性突变

• 批准号: 3281618
• 负责人: PC C. HUANG
• 金额: $ 20.68万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-01-01 至 1992-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3281618
• 关键词: chemical structure function; cyclic aminoacid; gene expression; genetic transcription; genetic translation; metallothionein; nucleic acid sequence; protein engineering; protein sequence

Site specific mutations in the metallothionein coding sequence will be followed for their expression. Selected codons will be specifically altered to give rise to amino acid replacement from non-aromatic to aromatic. The latter is normally absent in all native metallothioneins thus far analyzed. The possibility that the absence of aromatic amino acids is due to mutational probability of unfavorable processing at the transcriptional or translational level will be examined in vivo and in vitro. The altered metallothionein if obtained will be compared with the function and properties of those occurring naturally.

金属硫蛋白编码序列的位点特异性突变将被发现

项目成果

Purification of mammalian metallothionein from recombinant systems.

从重组系统中纯化哺乳动物金属硫蛋白。

• DOI: 10.1016/0076-6879(91)05113-a
• 发表时间: 1991
• 期刊: Methods in enzymology
• 作者: Cismowski,MJ;Huang,PC
• 通讯作者: Huang,PC

Primary sequence of duck metallothionein.

鸭金属硫蛋白的一级序列。

• DOI: 10.1016/0167-4838(90)90118-y
• 发表时间: 1990
• 期刊: Biochimica et biophysica acta
• 作者: Lin,LY;Liu,LF;Tam,MF;Huang,PC;Vestling,M;Fenselau,C
• 通讯作者: Fenselau,C

Intracellular metallothionein concentration and the rate of zinc or cadmium influx and MT mRNA accumulation in a CHO Cdr variant.

CHO Cdr 变体中细胞内金属硫蛋白浓度、锌或镉流入速率以及 MT mRNA 积累。

• DOI: 10.1016/0014-4827(89)90046-3
• 发表时间: 1989
• 期刊: Experimental cell research
• 影响因子: 3.7
• 作者: Morris,S;Huang,PC
• 通讯作者: Huang,PC

Age-dependent variation for inducibility of metallothionein genes in mouse liver by cadmium.

• DOI: 10.1002/dvg.1020090103
• 发表时间: 1988
• 期刊: Developmental genetics
• 作者: David J. Thomas;S. Morris;P. C. Huang

Pigeon metallothionein consists of two species.

鸽金属硫蛋白由两个物种组成。

• DOI: 10.1016/0167-4838(90)90175-f
• 发表时间: 1990
• 期刊: Biochimica et biophysica acta
• 作者: Lin,LY;Lin,WC;Huang,PC
• 通讯作者: Huang,PC