GENETIC ANALYSIS OF PROTEIN TRANSPORT INTO MITOCHONDRIA

蛋白质转运至线粒体的遗传分析

• 批准号: 3283776
• 负责人: Elton T. YOUNG
• 金额: $ 18.3万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1993-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3283776
• 关键词: Saccharomyces; aerobiosis; alcohol dehydrogenase; circular dichroism; drug resistance; fermentation; fungal genetics; genetic manipulation; genetic mapping; genetic recombination; isozymes; lethal genes; membrane proteins; microorganism metabolism; mitochondria; molecular cloning; mutant; nuclear magnetic resonance spectroscopy; nucleic acid sequence; protein sequence; protein signal sequence; protein transport; receptor

The role of the signal sequence in targeting a protein to its intracellular location will be studied by genetic, biochemical, and recombinant DNA methods. By using the yeast Saccharomyces we can take a genetic approach to identifying both elements of structure of the leader itself, and the cellular components with which it interacts. Mutants will be isolated by virtue of being unable to correctly localize a protein synthesized in the cytoplasm and destined for the mitochondria, or by virtue of being inviable due to a defect in protein targeting. The structure of a synthetic mitochondrial targeting sequence will be determined by two- dimensional nuclear magnetic resonance spectroscopy. Mutant presequences, defective in import or cleavage will be studied to correlate alterations in structure with alterations in function. The synthetic presequence will also be used as a probe to try to identify proteins on the mitochondria that act as receptor for targeting proteins to that organelle.

信号序列在蛋白质靶向其上的作用 细胞内的定位将通过遗传、生化和 重组DNA方法。通过使用酵母，我们可以 采用遗传学的方法来识别结构的两个元素 领导者本身，以及它与之相连的蜂窝组件 互动。变种人将被隔离，因为他们不能 正确定位在细胞质中合成的蛋白质并 注定是线粒体，或者是由于无法生存 与蛋白质靶向的缺陷有关。合成纤维的结构 线粒体的靶向序列将由两个- 三维核磁共振光谱学。突变型 对进口有缺陷或卵裂有缺陷的前测序进行研究 将结构的变化与功能的变化联系起来。 合成的前序列也将被用作探测器，试图 识别线粒体上作为受体的蛋白质 将蛋白质定位到细胞器。