GENETIC ANALYSIS OF PROTEIN TRANSPORT INTO MITOCHONDRIA

信号序列在将蛋白质靶向其 细胞内定位将通过遗传、生物化学和 重组DNA方法。 通过使用酵母菌，我们可以 采取遗传学的方法来识别结构的两个要素 领导者本身以及与之相关的细胞组件 互动。 突变体将被隔离，因为它们无法 正确定位在细胞质中合成的蛋白质， 或因其为无因，或因其为无因。 蛋白质定位的缺陷 一种合成的 线粒体靶向序列将由两个决定- 三维核磁共振波谱 突变体 将研究在输入或裂解中有缺陷的前序列， 将结构改变与功能改变联系起来。 合成的前序列也将被用作探针， 识别线粒体上作为受体的蛋白质， 将蛋白质定位到细胞器上

The role of the signal sequence in targeting a protein to its intracellular location will be studied by genetic, biochemical, and recombinant DNA methods. By using the yeast Saccharomyces we can take a genetic approach to identifying both elements of structure of the leader itself, and the cellular components with which it interacts. Mutants will be isolated by virtue of being unable to correctly localize a protein synthesized in the cytoplasm and destined for the mitochondria, or by virtue of being inviable due to a defect in protein targeting. The structure of a synthetic mitochondrial targeting sequence will be determined by two- dimensional nuclear magnetic resonance spectroscopy. Mutant presequences, defective in import or cleavage will be studied to correlate alterations in structure with alterations in function. The synthetic presequence will also be used as a probe to try to identify proteins on the mitochondria that act as receptor for targeting proteins to that organelle.