LOCALIZATION OF ADR1

ADR1 的本地化

• 批准号: 7957843
• 负责人: Elton T. YOUNG
• 金额: $ 1.63万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957843
• 关键词: 5' -AMP-activated protein kinase; Binding; Biology; Carbon; Cells; Computer Retrieval of Information on Scientific Projects Database; Ethanol; Fatty Acids; Fluorescence Microscopy; Funding; Fungal Genome; Gene Proteins; Genes; Genetic Transcription; Glucose; Glycerol; Grant; Institution; Mutation; Plasmids; Research; Research Personnel; Resources; Source; United States National Institutes of Health; Yeasts; Zinc Fingers; cell transformation; mutant; promoter; transcription factor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Adr1 is a carbon source-responsive zinc-finger transcription factor, required for transcription of a number of glucose-repressed genes (peroxisomal protein genes, genes required for ethanol, glycerol, and fatty acid utilization). Adr1 is present but inactive when cells are grown in the presence of glucose. Promoter binding by requires the AMP-activated protein kinase Snf1 which is phosphorylated and activated in the absence of glucose. Fluorescence microscopy of mutant yeast cells transformed with Adr1-GFP on a low-copy plasmid will be used to determine whether the mutations alter the intracellular distribution of Adr1-GFP.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 Adr1是一种碳源响应的锌指转录因子，需要转录一些葡萄糖抑制基因(过氧化物体蛋白基因，乙醇、甘油和脂肪酸利用所需的基因)。当细胞在葡萄糖存在下生长时，Adr1存在，但不活跃。启动子的结合需要AMP激活的蛋白激酶Snf1，它在没有葡萄糖的情况下被磷酸化并激活。在低拷贝质粒上转化Adr1-GFP的突变酵母细胞的荧光显微镜将被用来确定突变是否改变Adr1-GFP的细胞内分布。