MICROSTRUCTURAL HETEROGENEITY IN MEMBRANES
MICROSTRUCTURAL HETEROGENEITY IN MEMBRANES
批准号:
3281777
负责人:
Barry R Lentz
金额:
$7.39万
依托单位国家:
美国
项目类别:
财政年份:
1983
资助国家:
美国
项目状态:
已结题
起止时间:
1983-12-01 至 1986-11-30
中文摘要
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英文摘要
The aim of this proposal is to test and apply a new technique (fluorescence
lifetime heterogeneity analysis) for identifying the existence of
microheterogeneity in membrane structure. The power of lifetime
heterogeneity analysis is its potential sensitivity for detecting
short-lived membrane microdomains which do not appear as thermodynamically
distinct phases. The applicability of this technique will first be
evaluated in simple model membrane systems in which structural
heterogeneity is well established. Then, as initial applications, this
method will be used to test two hypotheses stemming from work of the
previous grant period: 1) that a trans-membrane protein both orders and
disorders its local lipid environment, and 2) that cholesterol induces
calorimetrically undetectable local domains in fluid lipid bilayers.
New applications will use lifetime analysis to detect microstructures
thought to be associated with two key cellular processes, membrane fusion
and phospholipid trans-membrane migration. In addition to fluorescence
lifetime analysis, appropriate thermodynamic techniques (scanning
calorimetry, steady-state probe fluorescence, chemical labeling,
freeze-fracture electron microscopy) will be used to examine bilayer
structural features associated with these events in well-defined, model
systems. In the case of polyethyleneglycol-induced membrane fusion, these
measurements will test the hypothesis that different structural
characteristics are associated with two crucial and previously inseparable
intermediate states in the fusion process: bilayer close apposition and
bilayer destabilization. In the case of cation-induced
phosphatidylglycerol transbilayer redistribution, results will test two
hypotheses: 1) transbilayer redistribution involves a two-component
interaction between a metal ion and a lipid bilayer and 2) this interaction
induces inverted micelle-like local structures in the lipid bilayer.
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Microstructural Heterogeneity in Membranes
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批准号:7869486
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项目类别:
-
资助金额:$9.88万
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财政年份:2009
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负责人:Barry R Lentz
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依托单位:
The Biophysical Society Summer Course of Biophysics
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批准号:7774371
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项目类别:
-
资助金额:$25.24万
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财政年份:2008
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负责人:Barry R Lentz
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依托单位:
The Biophysical Society Summer Course of Biophysics
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批准号:8078099
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项目类别:
-
资助金额:$9.52万
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财政年份:2008
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负责人:Barry R Lentz
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依托单位:
The Biophysical Society Summer Course of Biophysics
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批准号:8220808
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项目类别:
-
资助金额:$23.97万
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财政年份:2008
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负责人:Barry R Lentz
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依托单位:
The Biophysical Society Summer Course of Biophysics
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批准号:7570067
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项目类别:
-
资助金额:$24.51万
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财政年份:2008
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负责人:Barry R Lentz
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依托单位:
The Biophysical Society Summer Course of Biophysics
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批准号:7341285
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项目类别:
-
资助金额:$23.79万
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财政年份:2008
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负责人:Barry R Lentz
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依托单位:
Lipid Regulation of Thrombin Generation
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批准号:8269074
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项目类别:
-
资助金额:$36.63万
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财政年份:2004
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负责人:Barry R Lentz
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依托单位:
Lipid Regulation of Thrombin Generation
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批准号:8077274
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项目类别:
-
资助金额:$37.65万
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财政年份:2004
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负责人:Barry R Lentz
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依托单位:
Lipid Regulation of Thrombin Generation
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批准号:8113689
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项目类别:
-
资助金额:$3.42万
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财政年份:2004
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负责人:Barry R Lentz
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依托单位:
Lipid Regulation of Thrombin Generation
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批准号:7079288
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项目类别:
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资助金额:$32.08万
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财政年份:2004
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负责人:Barry R Lentz
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依托单位:
Lipid Regulation of Thrombin Generation
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批准号:6826058
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项目类别:
-
资助金额:$38.18万
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财政年份:2004
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负责人:Barry R Lentz
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依托单位:
Lipid Regulation of Thrombin Generation
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批准号:7248040
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项目类别:
-
资助金额:$31.15万
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财政年份:2004
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负责人:Barry R Lentz
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依托单位:
Lipid Regulation of Thrombin Generation
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批准号:7736810
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项目类别:
-
资助金额:$35.61万
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财政年份:2004
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负责人:Barry R Lentz
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依托单位:
Lipid Regulation of Thrombin Generation
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批准号:7914092
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项目类别:
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资助金额:$37.0万
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财政年份:2004
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负责人:Barry R Lentz
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依托单位:
Lipid Regulation of Thrombin Generation
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批准号:6911707
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项目类别:
-
资助金额:$32.85万
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财政年份:2004
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负责人:Barry R Lentz
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依托单位:
Molecular and Cellular Biophysics Training Program
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批准号:7071257
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项目类别:
-
资助金额:$22.16万
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财政年份:1995
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负责人:Barry R Lentz
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依托单位:
MOLECULAR AND CELLULAR BIOPHYSICS TRAINING PROGRAM
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批准号:6498482
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项目类别:
-
资助金额:$17.51万
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财政年份:1995
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负责人:Barry R Lentz
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依托单位:
Molecular and Cellular Biophysics Training Program
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批准号:8101279
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项目类别:
-
资助金额:$23.81万
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财政年份:1995
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负责人:Barry R Lentz
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依托单位:
MOLECULAR AND CELLULAR BIOPHYSICS TRAINING PROGRAM
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批准号:2331906
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项目类别:
-
资助金额:$11.14万
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财政年份:1995
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负责人:Barry R Lentz
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依托单位:
Molecular and Cellular Biophysics Training Program
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批准号:7861256
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项目类别:
-
资助金额:$23.54万
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财政年份:1995
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负责人:Barry R Lentz
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依托单位:
海外基金